ArticleThe Journal of cell biology1999
Growing pollen tubes possess a constitutive alkaline band in the clear zone and a growth-dependent acidic tip.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers, 1 of them a synthesis that pooled it.
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Who cites it
106 citing papers in PubMed, 1 synthesis or guideline pooled it, 305 citations in OpenAlex.
- Root hair growth from the pH point of view.Frontiers in plant science · 2022Pooled it
- Quantitative Profiling of Metabolites, Sugars, and Lipids in Pollen Tubes by Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2026Article
- SISE, free LabView-based software for ion flux measurements.Plant methods · 2025Article
- The Microbiome of Fertilization-Stage Maize Silks (Style) Encodes Genes and Expresses Traits That Potentially Promote Survival in Pollen/Style Niches and Host Reproduction.Microorganisms · 2024Article
- Computational design of Periplasmic binding protein biosensors guided by molecular dynamics.PLoS computational biology · 2024Article
- Differential sensitivity of ADF isovariants to a pH gradient promotes pollen tube growth.The Journal of cell biology · 2023Article
- Compounds composition of pollen tubes of Scots pine (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- SCaBP3/CBL7 negatively regulates the plasma membrane HPlant signaling & behavior · 2022Article
- Depletion plays a pivotal role in self-incompatibility, revealing a link between cellular energy status, cytosolic acidification and actin remodelling in pollen tubes.The New phytologist · 2022Article
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- Advances in Synthetic Fluorescent Probe Labeling for Live-Cell Imaging in Plants.Plant & cell physiology · 2021Review
- Two Is Company, but Four Is a Party-Challenges of Tetraploidization for Cell Wall Dynamics and Efficient Tip-Growth in Pollen.Plants (Basel, Switzerland) · 2021Review
- Pollen Germination and Pollen Tube Growth in Gymnosperms.Plants (Basel, Switzerland) · 2021Review
- Oxygen radicals and cytoplasm zoning in growing lily pollen tubes.Plant reproduction · 2021Article
- An optimized genetically encoded dual reporter for simultaneous ratio imaging of CaThe New phytologist · 2021Article
- The quest for the central players governing pollen tube growth and guidance.Plant physiology · 2021Review
- Measuring Extracellular Proton and Anionic Fluxes inBio-protocol · 2021Article
- ROS and Ions in Cell Signaling during Sexual Plant Reproduction.International journal of molecular sciences · 2020Review
- Dynamics of Pollen Activation and the Role of HPlants (Basel, Switzerland) · 2020Article
- Signalling Pinpointed to the Tip: The Complex Regulatory Network That Allows Pollen Tube Growth.Plants (Basel, Switzerland) · 2020Review
46 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using both the proton selective vibrating electrode to probe the extracellular currents and ratiometric wide-field fluorescence microscopy with the indicator 2', 7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF)-dextran to image the intracellular pH, we have examined the distribution and activity of protons (H+) associated with pollen tube growth. The intracellular images reveal that lily pollen tubes possess a constitutive alkaline band at the base of the clear zone and an acidic domain at the extreme apex. The extracellular observations, in close agreement, show a proton influx at the extreme apex of the pollen tube and an efflux in the region that corresponds to the position of the alkaline band. The ability to detect the intracellular pH gradient is strongly dependent on the concentration of exogenous buffers in the cytoplasm. Thus, even the indicator dye, if introduced at levels estimated to be of 1.0 microM or greater, will dissipate the gradient, possibly through shuttle buffering. The apical acidic domain correlates closely with the process of growth, and thus may play a direct role, possibly in facilitating vesicle movement and exocytosis. The alkaline band correlates with the position of the reverse fountain streaming at the base of the clear zone, and may participate in the regulation of actin filament formation through the modulation of pH-sensitive actin binding proteins. These studies not only demonstrate that proton gradients exist, but that they may be intimately associated with polarized pollen tube growth.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.