ArticleThe Journal of cell biology1999
Slow axonal transport of neurofilament protein in cultured neurons.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- Microglia-mediated demyelination protects against CD8Nature communications · 2023Article
- Dynamics of Vesicles Driven Into Closed Constrictions by Molecular Motors.Bulletin of mathematical biology · 2020Article
- Local Acceleration of Neurofilament Transport at Nodes of Ranvier.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Live-cell imaging of neurofilament transport in cultured neurons.Methods in cell biology · 2016Article
- Biomarkers of increased diffusion anisotropy in semi-acute mild traumatic brain injury: a longitudinal perspective.Brain : a journal of neurology · 2012Article
- Disruption of axonal transport in motor neuron diseases.International journal of molecular sciences · 2012Review
- Neurofilaments switch between distinct mobile and stationary states during their transport along axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Neurofilament polymer transport in axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Neurofilament transport is dependent on actin and myosin.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Abnormal neurofilament transport caused by targeted disruption of neuronal kinesin heavy chain KIF5A.The Journal of cell biology · 2003Article
- Rapid intermittent movement of axonal neurofilaments observed by fluorescence photobleaching.Molecular biology of the cell · 2001Article
- Neurofilaments consist of distinct populations that can be distinguished by C-terminal phosphorylation, bundling, and axonal transport rate in growing axonal neurites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Tubulin and neurofilament proteins are transported differently in axons of chicken motoneurons.Cellular and molecular neurobiology · 2000Article
- Neurofilaments are transported rapidly but intermittently in axons: implications for slow axonal transport.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Glutamate slows axonal transport of neurofilaments in transfected neurons.The Journal of cell biology · 2000Article
- Axonal transport of microtubule-associated protein 1B (MAP1B) in the sciatic nerve of adult rat: distinct transport rates of different isoforms.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Slow transport of unpolymerized tubulin and polymerized neurofilament in the squid giant axon.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have investigated the axonal transport of neurofilament protein in cultured neurons by constricting single axons with fine glass fibers. We observed a rapid accumulation of anterogradely and retrogradely transported membranous organelles on both sides of the constrictions and a more gradual accumulation of neurofilament protein proximal to the constrictions. Neurofilament protein accumulation was dependent on the presence of metabolic substrates and was blocked by iodoacetate, which is an inhibitor of glycolysis. These data indicate that neurofilament protein moves anterogradely in these axons by a mechanism that is directly or indirectly dependent on nucleoside triphosphates. The average transport rate was estimated to be at least 130 micrometer/h (3.1 mm/d), and approximately 90% of the accumulated neurofilament protein remained in the axon after detergent extraction, suggesting that it was present in a polymerized form. Electron microscopy demonstrated that there were an abnormally large number of neurofilament polymers proximal to the constrictions. These data suggest that the neurofilament proteins were transported either as assembled polymers or in a nonpolymeric form that assembled locally at the site of accumulation. This study represents the first demonstration of the axonal transport of neurofilament protein in cultured neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.