ArticlePlant physiology1999
Use of ubiquitin fusions to augment protein expression in transgenic plants.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Article
- Development of a tetracycline-inducible programmed ribosomal frameshifting platform for sensitive regulation of mammalian gene expression.Nucleic acids research · 2026Article
- Homeostatic regulation of ribosomal proteins by ubiquitin-independent cotranslational degradation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Optimization of Genome Knock-In Method: Search for the Most Efficient Genome Regions for Transgene Expression in Plants.International journal of molecular sciences · 2022Review
- Article
- Use of the LC3B-fusion technique for biochemical and structural studies of proteins involved in the N-degron pathway.The Journal of biological chemistry · 2020Article
- Expression of the Biologically Active Insulin Analog SCI-57 inFrontiers in pharmacology · 2019Article
- Stable production of cyanophycinase in Nicotiana benthamiana and its functionality to hydrolyse cyanophycin in the murine intestine.Plant biotechnology journal · 2017Article
- Expression of 16 Nitrogenase Proteins within the Plant Mitochondrial Matrix.Frontiers in plant science · 2017Article
- Translational fusion and redirection to thylakoid lumen as strategies to enhance accumulation of human papillomavirus E7 antigen in tobacco chloroplasts.Molecular biotechnology · 2014Article
- Use of Ubp1 protease analog to produce recombinant human growth hormone in Escherichia coli.Microbial cell factories · 2014Article
- The ubiquitination machinery of the ubiquitin system.The arabidopsis book · 2014Article
- Plant-produced human recombinant erythropoietic growth factors support erythroid differentiation in vitro.Stem cells and development · 2013Article
- Screening promoters for Anthurium transformation using transient expression.Plant cell reports · 2013Article
- Article
- Ubiquitin fusion expression and tissue-dependent targeting of hG-CSF in transgenic tobacco.BMC biotechnology · 2011Article
- Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation.BMC biotechnology · 2011Article
- Expression of a cholera toxin B subunit-neutralizing epitope of the porcine epidemic diarrhea virus fusion gene in transgenic lettuce (Lactuca sativa L.).Molecular biotechnology · 2011Article
- Hydrophobin fusions for high-level transient protein expression and purification in Nicotiana benthamiana.Plant physiology · 2010Article
- High expression level of a foot and mouth disease virus epitope in tobacco transplastomic plants.Planta · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major goal of plant biotechnology is the production of genetically engineered crops that express natural or foreign proteins at high levels. To enhance protein accumulation in transgenic plants, we developed a set of vectors that express proteins and peptides as C-terminal translational fusions with ubiquitin (UBQ). Studies of several proteins in tobacco (Nicotiana tabacum) showed that: (a) proteins can be readily expressed in plants as UBQ fusions; (b) by the action of endogenous UBQ-specific proteases (Ubps), these fusions are rapidly and precisely processed in vivo to release the fused protein moieties in free forms; (c) the synthesis of a protein as a UBQ fusion can significantly augment its accumulation; (d) proper processing and localization of a protein targeted to either the apoplast or the chloroplast is not affected by the N-terminal UBQ sequence; and (e) single amino acid substitutions surrounding the cleavage site can inhibit in vivo processing of the fusion by Ubps. Noncleavable UBQ fusions of beta-glucuronidase became extensively modified, with additional UBQs in planta. Because multiubiquitinated proteins are the preferred substrates of the 26S proteasome, noncleavable fusions may be useful for decreasing protein half-life. Based on their ability to augment protein accumulation and the sequence specificity of Ubps, UBQ fusions offer a versatile way to express plant proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.