ArticleMolecular biology of the cell1999
NF-kappaB and AP-1 activation by nitric oxide attenuated apoptotic cell death in RAW 264.7 macrophages.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 160 citations in OpenAlex.
- Gene regulatory logic of the interferon-β enhancer is characterized by two selectively deployed modes of transcription factor synergy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Ethanol Extract ofMolecules (Basel, Switzerland) · 2023Article
- Toxicity and virucidal activity of a neon-driven micro plasma jet on eukaryotic cells and a coronavirus.Free radical biology & medicine · 2022Article
- Structural and Functional Properties of Activator Protein-1 in Cancer and Inflammation.Evidence-based complementary and alternative medicine : eCAM · 2022Review
- Apremilast mitigates interleukin (IL)-13-induced inflammatory response and mucin production in human nasal epithelial cells (hNECs).Bioengineered · 2021Article
- Differential sensitivity of inflammatory macrophages and alternatively activated macrophages to ferroptosis.European journal of immunology · 2021Article
- Editorial: Genetic and Epigenetic Control of Immune Responses.Frontiers in immunology · 2021Article
- The Metabolic Signature of Macrophage Responses.Frontiers in immunology · 2019Review
- Role of PARP-1 as a novel transcriptional regulator of MMP-9 in diabetic retinopathy.Biochimica et biophysica acta. Molecular basis of disease · 2017Article
- Cytoplasmic translocation of high-mobility group box-1 protein is induced by diabetes and high glucose in retinal pericytes.Molecular medicine reports · 2016Article
- Anti-apoptotic mechanism of Bacoside rich extract against reactive nitrogen species induced activation of iNOS/Bax/caspase 3 mediated apoptosis in L132 cell line.Cytotechnology · 2014Article
- Neuroprotective and anti-apoptotic propensity of Bacopa monniera extract against sodium nitroprusside induced activation of iNOS, heat shock proteins and apoptotic markers in PC12 cells.Neurochemical research · 2014Article
- Sustained nitric oxide (NO)-releasing compound reverses dysregulated NO signal transduction in priapism.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Thioredoxin 1-mediated post-translational modifications: reduction, transnitrosylation, denitrosylation, and related proteomics methodologies.Antioxidants & redox signaling · 2011Review
- Curcumin: an anti-inflammatory molecule from a curry spice on the path to cancer treatment.Molecules (Basel, Switzerland) · 2011Review
- Cyclic GMP protects human macrophages against peroxynitrite-induced apoptosis.Journal of inflammation (London, England) · 2009Article
- Sumoylation of peroxisome proliferator-activated receptor gamma by apoptotic cells prevents lipopolysaccharide-induced NCoR removal from kappaB binding sites mediating transrepression of proinflammatory cytokines.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- NFkappaB in the mechanism of ammonia-induced astrocyte swelling in culture.Journal of neurochemistry · 2008Article
- Activation of c-Jun N-terminal kinase (JNK) signalling in experimentally induced gastric lesions in rats.Clinical and experimental immunology · 2006Article
- NF-kappaB and AP-1 connection: mechanism of NF-kappaB-dependent regulation of AP-1 activity.Molecular and cellular biology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A toxic dose of the nitric oxide (NO) donor S-nitrosoglutathione (GSNO; 1 mM) promoted apoptotic cell death of RAW 264.7 macrophages, which was attenuated by cellular preactivation with a nontoxic dose of GSNO (200 microM) or with lipopolysaccharide, interferon-gamma, and NG-monomethyl-L-arginine (LPS/IFN-gamma/NMMA) for 15 h. Protection from apoptosis was achieved by expression of cyclooxygenase-2 (Cox-2). Here we investigated the underlying mechanisms leading to Cox-2 expression. LPS/IFN-gamma/NMMA prestimulation activated nuclear factor (NF)-kappaB and promoted Cox-2 expression. Cox-2 induction by low-dose GSNO demanded activation of both NF-kappaB and activator protein-1 (AP-1). NF-kappaB supershift analysis implied an active p50/p65 heterodimer, and a luciferase reporter construct, containing four copies of the NF-kappaB site derived from the murine Cox-2 promoter, confirmed NF-kappaB activation after NO addition. An NF-kappaB decoy approach abrogated not only Cox-2 expression after low-dose NO or after LPS/IFN-gamma/NMMA but also inducible protection. The importance of AP-1 for Cox-2 expression and cell protection by low-level NO was substantiated by using the extracellular signal-regulated kinase inhibitor PD98059, blocking NO-elicited Cox-2 expression, but leaving the cytokine signal unaltered. Transient transfection of a dominant-negative c-Jun mutant further attenuated Cox-2 expression by low-level NO. Whereas cytokine-mediated Cox-2 induction relies on NF-kappaB activation, a low-level NO-elicited Cox-2 response required activation of both NF-kappaB and AP-1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.