ArticleMolecular biology of the cell1999
Cleavage furrows formed between centrosomes lacking an intervening spindle and chromosomes contain microtubule bundles, INCENP, and CHO1 but not CENP-E.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 77 citations in OpenAlex.
- Functional midbody assembly in the absence of a central spindle.The Journal of cell biology · 2022Article
- Review
- Lateral attachment of kinetochores to microtubules is enriched in prometaphase rosette and facilitates chromosome alignment and bi-orientation establishment.Scientific reports · 2018Article
- Spindle-to-Cortex Communication in Cleaving Frog Eggs.Cold Spring Harbor symposia on quantitative biology · 2017Article
- The Timing of Midzone Stabilization during Cytokinesis Depends on Myosin II Activity and an Interaction between INCENP and Actin.Current biology : CB · 2016Article
- Spindle-to-cortex communication in cleaving, polyspermic Xenopus eggs.Molecular biology of the cell · 2015Article
- Centralspindlin and chromosomal passenger complex behavior during normal and Rappaport furrow specification in echinoderm embryos.Cytoskeleton (Hoboken, N.J.) · 2012Article
- CAMP (C13orf8, ZNF828) is a novel regulator of kinetochore-microtubule attachment.The EMBO journal · 2011Article
- Aurora B and 14-3-3 coordinately regulate clustering of centralspindlin during cytokinesis.Current biology : CB · 2010Article
- Kinesins to the core: The role of microtubule-based motor proteins in building the mitotic spindle midzone.Seminars in cell & developmental biology · 2010Review
- Self-organization of intracellular gradients during mitosis.Cell division · 2010Article
- Cytokinetic astralogy.The Journal of cell biology · 2009Article
- The 3Ms of central spindle assembly: microtubules, motors and MAPs.Nature reviews. Molecular cell biology · 2009Review
- The localization of inner centromeric protein (INCENP) at the cleavage furrow is dependent on Kif12 and involves interactions of the N terminus of INCENP with the actin cytoskeleton.Molecular biology of the cell · 2007Article
- Deregulation of HEF1 impairs M-phase progression by disrupting the RhoA activation cycle.Molecular biology of the cell · 2006Article
- Contractile ring-independent localization of DdINCENP, a protein important for spindle stability and cytokinesis.Molecular biology of the cell · 2006Article
- Taxol-stabilized microtubules can position the cytokinetic furrow in mammalian cells.Molecular biology of the cell · 2005Article
- Regulation of cell cycle by the anaphase spindle midzone.BMC cell biology · 2004Article
- Review
- Rappaport furrows on our minds: the ASCB Cytokinesis Meeting Burlington, VT July 22-25, 2004.The Journal of cell biology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
PtK1 cells containing two independent mitotic spindles can cleave between neighboring centrosomes, in the absence of an intervening spindle, as well as at the spindle equators. We used same-cell video, immunofluorescence, and electron microscopy to compare the structure and composition of normal equatorial furrows with that of ectopic furrows formed between spindles. As in controls, ectopic furrows contained midbodies composed of microtubule bundles and an electron-opaque matrix. Despite the absence of an intervening spindle and chromosomes, the midbodies associated with ectopic furrows also contained the microtubule-bundling protein CHO1 and the chromosomal passenger protein INCENP. However, CENP-E, another passenger protein, was not found in ectopic furrows but was always present in controls. We also examined cells in which the ectopic furrow initiated but relaxed. Although relaxing furrows contained overlapping microtubules from opposing centrosomes, they lacked microtubule bundles as well as INCENP and CHO1. Together these data suggest that the mechanism defining the site of furrow formation during mitosis in vertebrates does not depend on the presence of underlying microtubule bundles and chromosomes or on the stable association of INCENP or CHO1. The data also suggest that the completion of cytokinesis requires the presence of microtubule bundles and specific proteins (e.g., INCENP, CHO1, etc.) that do not include CENP-E.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.