ArticleMolecular biology of the cell1999
The multiple roles of Cyk1p in the assembly and function of the actomyosin ring in budding yeast.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Central Role of the Actomyosin Ring in Coordinating Cytokinesis Steps in Budding Yeast.Journal of fungi (Basel, Switzerland) · 2024Review
- Unraveling the mechanisms and evolution of a two-domain module in IQGAP proteins for controlling eukaryotic cytokinesis.Cell reports · 2023Article
- PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis.Cellular and molecular life sciences : CMLS · 2022Article
- Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome Biogenesis.Biomedicines · 2022Article
- Single-molecule imaging of IQGAP1 regulating actin filament dynamics.Molecular biology of the cell · 2022Article
- Article
- Review
- Distinct Roles of Myosin-II Isoforms in Cytokinesis under Normal and Stressed Conditions.iScience · 2019Article
- Budding YeastG3 (Bethesda, Md.) · 2018Article
- Role of the Hof1-Cyk3 interaction in cleavage-furrow ingression and primary-septum formation during yeast cytokinesis.Molecular biology of the cell · 2018Article
- Mechanics and regulation of cytokinesis in budding yeast.Seminars in cell & developmental biology · 2017Review
- The final cut: cell polarity meets cytokinesis at the bud neck in S. cerevisiae.Cellular and molecular life sciences : CMLS · 2016Review
- Actomyosin ring driven cytokinesis in budding yeast.Seminars in cell & developmental biology · 2016Review
- Computational model of polarized actin cables and cytokinetic actin ring formation in budding yeast.Cytoskeleton (Hoboken, N.J.) · 2015Article
- Dephosphorylation of Iqg1 by Cdc14 regulates cytokinesis in budding yeast.Molecular biology of the cell · 2015Article
- IQGAP1: insights into the function of a molecular puppeteer.Molecular immunology · 2015Review
- The biology of IQGAP proteins: beyond the cytoskeleton.EMBO reports · 2015Review
- IQGAP1 is associated with nuclear envelope reformation and completion of abscission.Cell cycle (Georgetown, Tex.) · 2015Article
- Cdk1-dependent phosphorylation of Iqg1 governs actomyosin ring assembly prior to cytokinesis.Journal of cell science · 2014Article
- Article
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The budding yeast IQGAP-like protein Cyk1p/Iqg1p localizes to the mother-bud junction during anaphase and has been shown to be required for the completion of cytokinesis. In this study, video microscopy analysis of cells expressing green fluorescent protein-tagged Cyk1p/Iqg1p demonstrates that Cyk1p/Iqg1p is a dynamic component of the contractile ring during cytokinesis. Furthermore, in the absence of Cyk1p/Iqg1p, myosin II fails to undergo the contraction-like size change at the end of mitosis. To understand the mechanistic role of Cyk1p/Iqg1p in actomyosin ring assembly and dynamics, we have investigated the role of the structural domains that Cyk1p/Iqg1p shares with IQGAPs. An amino terminal portion containing the calponin homology domain binds to actin filaments and is required for the assembly of actin filaments to the ring. This result supports the hypothesis that Cyk1p/Iqg1p plays a direct role in F-actin recruitment. Deletion of the domain harboring the eight IQ motifs abolishes the localization of Cyk1p/Iqg1p to the bud neck, suggesting that Cyk1p/Iqg1p may be localized through interactions with a calmodulin-like protein. Interestingly, deletion of the COOH-terminal GTPase-activating protein-related domain does not affect Cyk1p/Iqg1p localization or actin recruitment to the ring but prevents actomyosin ring contraction. In vitro binding experiments show that Cyk1p/Iqg1p binds to calmodulin, Cmd1p, in a calcium-dependent manner, and to Tem1p, a small GTP-binding protein previously found to be required for the completion of anaphase. These results demonstrate the critical function of Cyk1p/Iqg1p in regulating various steps of actomyosin ring assembly and cytokinesis.
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