ArticleProceedings of the National Academy of Sciences of the United States of America1999
Isometric contraction induces the Ca2+-independent activation of the endothelial nitric oxide synthase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 114 citations in OpenAlex.
- Decreased morbidity following long saphenous vein harvesting using a minimally invasive technique: a randomised controlled trial comparing two techniques for long saphenous vein harvest.Journal of cardiothoracic surgery · 2006Trial
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- Endothelium-derived 6-nitrodopamine is the major mechanism by which nitric oxide relaxes the rabbit isolated aorta.Frontiers in pharmacology · 2024Article
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- Potentiation of Acetylcholine-Induced Relaxation of Aorta in Male UC Davis Type 2 Diabetes Mellitus (UCD-T2DM) Rats: Sex-Specific Responses.Frontiers in physiology · 2021Article
- Enzyme-mediated nitric oxide production in vasoactive erythrocyte membrane-enclosed coacervate protocells.Nature chemistry · 2020Article
- Stimulation history affects vasomotor responses in rat mesenteric arterioles.Pflugers Archiv : European journal of physiology · 2019Article
- Cell chirality regulates intercellular junctions and endothelial permeability.Science advances · 2018Article
- Inwardly rectifying KThe Journal of physiology · 2017Article
- Buckling Reduces eNOS Production and Stimulates Extracellular Matrix Remodeling in Arteries in Organ Culture.Annals of biomedical engineering · 2016Article
- Exercise training improves vascular mitochondrial function.American journal of physiology. Heart and circulatory physiology · 2016Article
- Genipin Derivatives Protect RGC-5 from Sodium Nitroprusside-Induced Nitrosative Stress.International journal of molecular sciences · 2016Article
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- Roles of inflammation, oxidative stress, and vascular dysfunction in hypertension.BioMed research international · 2014Review
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- Article
- Differential sensitivity of basal and acetylcholine-induced activity of nitric oxide to blockade by asymmetric dimethylarginine in the rat aorta.British journal of pharmacology · 2010Article
- Myoendothelial coupling in the mesenteric arterial bed; segmental differences and interplay between nitric oxide and endothelin-1.British journal of pharmacology · 2009Article
- Effects of chronic in vivo administration of nitroglycerine on ACh-induced endothelium-dependent relaxation in rabbit cerebral arteries.British journal of pharmacology · 2008Article
- Resting tension affects eNOS activity in a calcium-dependent way in airways.Mediators of inflammation · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shear stress and tyrosine phosphatase inhibitors have been shown to activate the endothelial NO synthase (eNOS) in a Ca2+/calmodulin-independent manner. We report here that isometric contraction of rabbit aorta activates eNOS by a pharmacologically identical pathway. Endothelium-intact aortic rings were precontracted under isometric conditions up to 60% of the maximal phenylephrine-induced tone. The NO synthase inhibitor NGnitro-L-arginine (L-NA) and the soluble guanylyl cyclase inhibitor NS 2028 induced an additional contraction, the amplitude of which depended on the level of precontraction. The maximal production of NO by isometrically contracted aortic rings (as estimated by the increase in cGMP in detector smooth muscle cells in a superfusion bioassay) was observed during the initial phase of isometric contraction and was greater than that detected following the application of acetylcholine. The supplementary L-NA-induced increase in vascular tone was inhibited by the nonselective kinase inhibitor staurosporine and the tyrosine kinase inhibitors erbstatin A and herbimycin A. Another tyrosine kinase inhibitor, genistein, the calmodulin antagonist calmidazolium, and the selective protein kinase C inhibitor, Ro 31-8220, had no effect. Coincident with the enhanced NO formation during isometric contraction was an increase in the tyrosine phosphorylation of endothelial proteins, which also correlated with the level of precontraction. Thus, isometric contraction activates eNOS via a Ca2+-independent, tyrosine kinase inhibitor-sensitive pathway and, like shear stress, seems to be an independent determinant of mechanically induced NO formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.