ArticleProceedings of the National Academy of Sciences of the United States of America1999
Multiple and diverse forms of regulated exocytosis in wild-type and defective PC12 cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 59 citations in OpenAlex.
- In vivo single-molecule imaging of syntaxin1A reveals polyphosphoinositide- and activity-dependent trapping in presynaptic nanoclusters.Nature communications · 2017Article
- Systematic spatial mapping of proteins at exocytic and endocytic structures.Molecular biology of the cell · 2014Article
- Article
- In PC12 cells, expression of neurosecretion and neurite outgrowth are governed by the transcription repressor REST/NRSF.Cellular and molecular neurobiology · 2010Review
- Fusion gains independence.The Journal of general physiology · 2008Article
- Annexin2 coating the surface of enlargeosomes is needed for their regulated exocytosis.The EMBO journal · 2006Article
- Sequential compound exocytosis of large dense-core vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis.The Journal of physiology · 2005Article
- Exocytosis and endocytosis of small vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis.The Journal of physiology · 2005Article
- EGFP-tagged vasopressin precursor protein sorting into large dense core vesicles and secretion from PC12 cells.Cellular and molecular neurobiology · 2005Article
- Enlargeosome, an exocytic vesicle resistant to nonionic detergents, undergoes endocytosis via a nonacidic route.Molecular biology of the cell · 2004Article
- Quantal release of ATP from clusters of PC12 cells.The Journal of physiology · 2004Article
- The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing.EMBO reports · 2004Article
- Lysosomes and the plasma membrane: trypanosomes reveal a secret relationship.The Journal of cell biology · 2002Review
- Ion selectivities of the Ca(2+) sensors for exocytosis in rat phaeochromocytoma cells.The Journal of physiology · 2001Article
- Sorting to synaptic-like microvesicles from early and late endosomes requires overlapping but not identical targeting signals.Molecular biology of the cell · 2000Article
- Synaptotagmin VII regulates Ca(2+)-dependent exocytosis of lysosomes in fibroblasts.The Journal of cell biology · 2000Article
- Neurosecretory cells without neurosecretion: evidence of an independently regulated trait of the cell phenotype.The Journal of physiology · 1999Review
- Surface wound healing: a new, general function of eukaryotic cells.Journal of cellular and molecular medicineReview
- Patching the membranes up - new views on cellular healing.Journal of cellular and molecular medicineArticle
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulated exocytosis triggered by the photolysis of a caged Ca2+ compound, DM-nitrophen, was investigated by patch-clamp capacitance measurements in two clones of PC12, the first wild-type and the second (PC12-27) defective of both types of classical secretory vesicles together with the neuronal-type receptors for the attachment proteins of the N-ethylmaleimide-sensitive fusion protein, the so called SNAREs. Moreover, the electrophysiological data were correlated with the ultrastructure of resting quick-frozen-freeze-dried cells of the two clones. Wild-type PC12 exhibited two-component capacitance responses, time constants of 30-100 ms and >10 s, that previous studies had suggested to reflect primarily the fusion of the small and large secretory vesicles, each contributing cell surface increases of approximately 10%. Both of these components were largely and specifically inhibited whether cells previously were microinjected with tetanus toxin light chain. In the defective clone, large responses also were recorded ( approximately 19% surface expansion; time constant, approximately 1 s) that, in contrast to those of the wild-type, were entirely resistant to the toxin. Although secretory organelles, i.e., large vesicles and also profiles of small vesicles, were abundant at the cell periphery and often docked to the plasmalemma of resting wild-type PC12, in the defective clone, no superficial accumulation of vesicles was observed. Our coordinate structural and functional results have revealed diversities between the two classical forms of regulated secretion in wild-type PC12 and have provided evidence of a toxin-insensitive form of Ca2+-induced exocytosis, prominent in the defective clone, that may play an important role(s) in cellular physiology.
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