ArticleProceedings of the National Academy of Sciences of the United States of America1999
The nuclear matrix prepared by amine modification.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 55 citations in OpenAlex.
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- Variant Ciz1 is a circulating biomarker for early-stage lung cancer.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Nuclear routing networks span between nuclear pore complexes and genomic DNA to guide nucleoplasmic trafficking of biomolecules.Journal of fertilization in vitro · 2012Article
- Routing of Biomolecules and Transgenes' Vectors in Nuclei of Oocytes.Journal of fertilization in vitro · 2012Article
- An architectural genetic and epigenetic perspective.Integrative biology : quantitative biosciences from nano to macro · 2011Review
- Silencing by nuclear matrix attachment distinguishes cell-type specificity: association with increased proliferation capacity.Nucleic acids research · 2009Article
- A novel form of the telomere-associated protein TIN2 localizes to the nuclear matrix.Cell cycle (Georgetown, Tex.) · 2009Article
- Maintenance of a functional higher order chromatin structure: The role of the nuclear matrix in normal and disease states.Gene therapy & molecular biology · 2009Article
- Identifying functional neighborhoods within the cell nucleus: proximity analysis of early S-phase replicating chromatin domains to sites of transcription, RNA polymerase II, HP1gamma, matrin 3 and SAF-A.Journal of cellular biochemistry · 2008Article
- NMPdb: Database of Nuclear Matrix Proteins.Nucleic acids research · 2005Article
- From DNA structure to gene expression: mediators of nuclear compartmentalization and dynamics.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Review
- RGS12TS-S localizes at nuclear matrix-associated subnuclear structures and represses transcription: structural requirements for subnuclear targeting and transcriptional repression.Molecular and cellular biology · 2002Article
- Regulation and localization of the Bloom syndrome protein in response to DNA damage.The Journal of cell biology · 2001Article
- Use of matrix attachment regions (MARs) to minimize transgene silencing.Plant molecular biology · 2000Review
- Spatial organization of RNA polymerase II transcription in the nucleus.Nucleic acids research · 2000Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The nucleus is spatially ordered by attachments to a nonchromatin nuclear structure, the nuclear matrix. The nuclear matrix and chromatin are intimately connected and integrated structures, and so a major technical challenge in nuclear matrix research has been to remove chromatin while retaining a native nuclear matrix. Most methods for removing chromatin require first a nuclease digestion and then a salt extraction to remove cut chromatin. We have hypothesized that cut chromatin is held in place by charge interactions involving nucleosomal amino groups. We have tested this hypothesis by chemically modifying amino groups after nuclease digestion. By using this protocol, chromatin could be effectively removed at physiological ionic strength. We compared the ultrastructure and composition of this nuclear matrix preparation with the traditional high-salt nuclear matrix and with the third nuclear matrix preparation that we have developed from which chromatin is removed after extensive crosslinking. All three matrix preparations reveal internal nuclear matrix structures that are built on a network of branched filaments of about 10 nm diameter. That such different chromatin-removal protocols reveal similar principles of nuclear matrix construction increases our confidence that we are observing important architectural elements of the native structure in the living cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.