ArticleProceedings of the National Academy of Sciences of the United States of America1999
SEM1, a homologue of the split hand/split foot malformation candidate gene Dss1, regulates exocytosis and pseudohyphal differentiation in yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 56 citations in OpenAlex.
- DSS1 is required for proper Integrator-PP2A function.Nature communications · 2025Article
- CRISPR/Cas9-Targeted Disruption of Two Highly HomologousInternational journal of molecular sciences · 2023Article
- Yeast as a Model to Unravel New BRCA2 Functions in Cell Metabolism.Frontiers in oncology · 2022Article
- The disordered PCI-binding human proteins CSNAP and DSS1 have diverged in structure and function.Protein science : a publication of the Protein Society · 2021Article
- Intrinsically disordered protein AtDSS1(V) participates in plant defense response to oxidative stress.Protoplasma · 2021Article
- Ubiquitin-proteasome-mediated cyclin C degradation promotes cell survival following nitrogen starvation.Molecular biology of the cell · 2020Article
- Article
- DSS1 interacts with and stimulates RAD52 to promote the repair of DSBs.Nucleic acids research · 2020Article
- Expanded Interactome of the Intrinsically Disordered Protein Dss1.Cell reports · 2018Article
- Sem1 links proteasome stability and specificity to multicellular development.PLoS genetics · 2018Article
- The exocyst subunit Sec3 is regulated by a protein quality control pathway.The Journal of biological chemistry · 2017Article
- A Novel Frizzled-Based Screening Tool Identifies Genetic Modifiers of Planar Cell Polarity in Drosophila Wings.G3 (Bethesda, Md.) · 2016Article
- A bacterial genetic selection system for ubiquitylation cascade discovery.Nature methods · 2016Article
- Article
- The over-expression of the β2 catalytic subunit of the proteasome decreases homologous recombination and impairs DNA double-strand break repair in human cells.Journal of biomedicine & biotechnology · 2011Article
- Functional analysis of phosphorylation on Saccharomyces cerevisiae syntaxin 1 homologues Sso1p and Sso2p.PloS one · 2010Article
- Split hand/foot malformation due to chromosome 7q aberrations(SHFM1): additional support for functional haploinsufficiency as the causative mechanism.European journal of human genetics : EJHG · 2009Article
- Sem1 is a functional component of the nuclear pore complex-associated messenger RNA export machinery.The Journal of cell biology · 2009Article
- C. elegans dss-1 is functionally conserved and required for oogenesis and larval growth.BMC developmental biology · 2008Article
- The Princeton Protein Orthology Database (P-POD): a comparative genomics analysis tool for biologists.PloS one · 2007Article
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Authors and funding
5 authors.
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Abstract
The exocyst is an essential multiprotein complex mediating polarized secretion in yeast. Here we describe a gene, SEM1, that can multicopy-suppress exocyst mutants sec3-2, sec8-9, sec10-2, and sec15-1. SEM1 is highly conserved among eukaryotic species. Its human homologue, DSS1, has been suggested as a candidate gene for the split hand/split foot malformation disorder. SEM1 is not an essential gene. However, its deletion rescued growth of the temperature-sensitive exocyst mutants sec3-2, sec8-9, sec10-1, and sec15-1 at the restrictive temperature. Cell fractionation showed that Sem1p is mainly cytosolic but also associates with the microsomal fraction. In linear sucrose gradients, Sem1p cosedimented with the exocyst component Sec8p. In diploid cells that normally do not form pseudohyphae (S288C background), deletion of SEM1 triggered pseudohyphal growth. This phenotype was abolished after reintroduction of either SEM1 or the mouse homologue Dss1 into the cells. In diploids that have normal capacity for pseudohyphal growth (Sigma1278b background), deletion of SEM1 enhanced filamentous growth. The functionality of both SEM1 and Dss1 in a differentiation process in yeast suggests that Dss1 indeed could be the gene affected in the split hand/split foot malformation disorder. These results characterize SEM1 as a regulator of both exocyst function and pseudohyphal differentiation and suggest a unique link between these two cellular functions in yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.