ArticleProceedings of the National Academy of Sciences of the United States of America1999
The specificity of interaction of archaeal transducers with their cognate sensory rhodopsins is determined by their transmembrane helices.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 64 citations in OpenAlex.
- Opsin-Mediated Inhibition of Bacterioruberin Synthesis in Halophilic Archaea.Journal of bacteriology · 2017Article
- Microbial rhodopsins: wide distribution, rich diversity and great potential.Biophysics and physicobiology · 2015Review
- A predictive computational model of the kinetic mechanism of stimulus-induced transducer methylation and feedback regulation through CheY in archaeal phototaxis and chemotaxis.BMC systems biology · 2010Article
- Protein-protein interaction changes in an archaeal light-signal transduction.Journal of biomedicine & biotechnology · 2010Article
- Different dark conformations function in color-sensitive photosignaling by the sensory rhodopsin I-HtrI complex.Biophysical journal · 2007Article
- Role of the cytoplasmic domain in Anabaena sensory rhodopsin photocycling: vectoriality of Schiff base deprotonation.Biophysical journal · 2006Article
- Computational analysis of the transient movement of helices in sensory rhodopsin II.Protein science : a publication of the Protein Society · 2005Article
- Time-resolved detection of sensory rhodopsin II-transducer interaction.Biophysical journal · 2004Article
- Article
- Role of Arg-72 of pharaonis Phoborhodopsin (sensory rhodopsin II) on its photochemistry.Biophysical journal · 2004Article
- A novel mode of sensory transduction in archaea: binding protein-mediated chemotaxis towards osmoprotectants and amino acids.The EMBO journal · 2002Article
- An archaeal photosignal-transducing module mediates phototaxis in Escherichia coli.Journal of bacteriology · 2001Article
- X-ray structure of sensory rhodopsin II at 2.1-A resolution.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Crystal structure of sensory rhodopsin II at 2.4 angstroms: insights into color tuning and transducer interaction.Science (New York, N.Y.) · 2001Article
- Electrophysiological characterization of specific interactions between bacterial sensory rhodopsins and their transducers.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Genomic perspective on the photobiology of Halobacterium species NRC-1, a phototrophic, phototactic, and UV-tolerant haloarchaeon.Photosynthesis research · 2001Article
- Structure of a conserved receptor domain that regulates kinase activity: the cytoplasmic domain of bacterial taxis receptors.Current opinion in structural biology · 2000Review
- A functional-phylogenetic classification system for transmembrane solute transporters.Microbiology and molecular biology reviews : MMBR · 2000Review
- Proton circulation during the photocycle of sensory rhodopsin II.Biophysical journal · 1999Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Chimeras of the Halobacterium salinarum transducers HtrI and HtrII were constructed to study the structural determinants for their specific interaction with the phototaxis receptors sensory rhodopsins I and II (SRI and SRII), respectively. Interaction of receptors and transducers was assessed by two criteria: phototaxis responses by the cells and transducer-modulation of receptor photochemical reaction kinetics in membranes. Coexpression of HtrI with SRII or HtrII with SRI did not result in interaction by either criterion. Each receptor was coexpressed with chimeric transducers in which various domains of the two transducers were interchanged. The results show that the presence of the two transmembrane helices of HtrI in a chimera is necessary and sufficient for functional transducer complexation with SRI, i.e., for wild-type SRI photoreactions and attractant and 2-photon repellent phototaxis responses. Additionally, a previously demonstrated chaperone-like facilitation of SRI folding or stability by HtrI was shown to depend only on the two transmembrane helices of HtrI in chimeric transducers. Similarly, the two transmembrane helices of HtrII specify interaction with the repellent receptor SRII according to motility analysis and laser-flash spectroscopy. The results support a model in which the membrane domains of the receptor/transducer complexes, consisting of the seven helices of the receptor interacting with the four-helix bundle of the transducer dimer, produce SRI- and SRII-specific signals to the flagellar motor by means of interchangeable cytoplasmic domains.
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