ArticleGenetics1999
The gene search system. A method for efficient detection and rapid molecular identification of genes in Drosophila melanogaster.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
94 citing papers in PubMed, 232 citations in OpenAlex.
- A gain-of-function screening reveals dAnkmy2 as a potential mediator of lifespan extension and oxidative stress resistance in Drosophila melanogaster.Biogerontology · 2026Article
- Plexin/Semaphorin antagonism orchestrates collective cell migration and organ sculpting by regulating epithelial-mesenchymal balance.Science advances · 2025Article
- Dominant suppressor genes of p53-induced apoptosis in Drosophila melanogaster.G3 (Bethesda, Md.) · 2024Article
- Protein retention in the endoplasmic reticulum rescues Aβ toxicity in Drosophila.Neurobiology of aging · 2023Article
- The Drosophila AWP1 ortholog Doctor No regulates JAK/STAT signaling for left-right asymmetry in the gut by promoting receptor endocytosis.Development (Cambridge, England) · 2023Article
- Septins tune lipid kinase activity and PI(4,5)PLife science alliance · 2022Article
- The need for unbiased genetic screens to dissect aggression inFrontiers in behavioral neuroscience · 2022Review
- Innovation of heterochromatin functions drives rapid evolution of essential ZAD-ZNF genes ineLife · 2020Article
- Abnormal Social Interactions in aInternational journal of molecular sciences · 2020Article
- Anchor Away - A Fast, Reliable and Reversible Technique To Inhibit Proteins inG3 (Bethesda, Md.) · 2020Article
- Antioxidant Therapy in Parkinson's Disease: Insights fromAntioxidants (Basel, Switzerland) · 2020Review
- Maelstrom Represses Canonical Polymerase II Transcription within Bi-directional piRNA Clusters in Drosophila melanogaster.Molecular cell · 2019Article
- Partial proteasomal degradation of Lola triggers the male-to-female switch of a dimorphic courtship circuit.Nature communications · 2019Article
- Using Drosophila Models and Tools to Understand the Mechanisms of Novel Human Cancer Driver Gene Function.Advances in experimental medicine and biology · 2019Review
- Article
- Ubiquitin-Binding Protein CG5445 Suppresses Aggregation and Cytotoxicity of Amyotrophic Lateral Sclerosis-Linked TDP-43 in Drosophila.Molecular and cellular biology · 2018Article
- The core-promoter factor TRF2 mediates a Fruitless action to masculinize neurobehavioral traits in Drosophila.Nature communications · 2017Article
- Regulatory Mechanisms of Metamorphic Neuronal Remodeling Revealed Through a Genome-Wide Modifier Screen inGenetics · 2017Article
- Taking Stock of theGenetics · 2017Review
- Stuxnet Facilitates the Degradation of Polycomb Protein during Development.Developmental cell · 2016Article
34 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have constructed a P-element-based gene search vector for efficient detection of genes in Drosophila melanogaster. The vector contains two copies of the upstream activating sequence (UAS) enhancer adjacent to a core promoter, one copy near the terminal inverted repeats at each end of the vector, and oriented to direct transcription outward. Genes were detected on the basis of phenotypic changes caused by GAL4-dependent forced expression of vector-flanking DNA, and the transcripts were identified with reverse transcriptase PCR (RT-PCR) using the vector-specific primer and followed by direct sequencing. The system had a greater sensitivity than those already in use for gain-of-function screening: 64% of the vector insertion lines (394/613) showed phenotypes with forced expression of vector-flanking DNA, such as lethality or defects in adult structure. Molecular analysis of 170 randomly selected insertions with forced expression phenotypes revealed that 21% matched the sequences of cloned genes, and 18% matched reported expressed sequence tags (ESTs). Of the insertions in cloned genes, 83% were upstream of the protein-coding region. We discovered two new genes that showed sequence similarity to human genes, Ras-related protein 2 and microsomal glutathione S-transferase. The system can be useful as a tool for the functional mapping of the Drosophila genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.