ArticleGenetics1999
A highly conserved sequence in the 3'-untranslated region of the drosophila Adh gene plays a functional role in Adh expression.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- MicroSalmon: A Comprehensive, Searchable Resource of Predicted MicroRNA Targets and 3'UTR Cis-Regulatory Elements in the Full-Length Sequenced Atlantic Salmon Transcriptome.Non-coding RNA · 2021Article
- New insights into the catalytic inactivity of mammary gland protein-40, a chitinase-like protein expressed during mammary gland involution.Molecular biology reports · 2019Article
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- Evolutionary dynamics of a conserved sequence motif in the ribosomal genes of the ciliate Paramecium.BMC evolutionary biology · 2010Article
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- In silico investigations on functional and haplotype tag SNPs associated with congenital long QT syndromes (LQTSs).Genomic medicine · 2008Article
- Conflicting selection pressures on synonymous codon use in yeast suggest selection on mRNA secondary structures.BMC evolutionary biology · 2008Article
- Functional analysis of Drosophila melanogaster gene regulatory sequences by transgene coplacement.Genetics · 2004Article
- Pleiotropic effect of disrupting a conserved sequence involved in a long-range compensatory interaction in the Drosophila Adh gene.Genetics · 2004Article
- Compensatory evolution of a precursor messenger RNA secondary structure in the Drosophila melanogaster Adh gene.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- UTRdb and UTRsite: specialized databases of sequences and functional elements of 5' and 3' untranslated regions of eukaryotic mRNAs. Update 2002.Nucleic acids research · 2002Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Phylogenetic analysis identified a highly conserved eight-base sequence (AAGGCTGA) within the 3'-untranslated region (UTR) of the Drosophila alcohol dehydrogenase gene, Adh. To examine the functional significance of this conserved motif, we performed in vitro deletion mutagenesis on the D. melanogaster Adh gene followed by P-element-mediated germline transformation. Deletion of all or part of the eight-base sequence leads to a twofold increase in in vivo ADH enzymatic activity. The increase in activity is temporally and spatially general and is the result of an underlying increase in Adh transcript. These results indicate that the conserved 3'-UTR motif plays a functional role in the negative regulation of Adh gene expression. The evolutionary significance of our results may be understood in the context of the amino acid change that produces the ADH-F allele and also leads to a twofold increase in ADH activity. While there is compelling evidence that the amino acid replacement has been a target of positive selection, the conservation of the 3'-UTR sequence suggests that it is under strong purifying selection. The selective difference between these two sequence changes, which have similar effects on ADH activity, may be explained by different metabolic costs associated with the increase in activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.