ArticleGenetics1999
A mutation in the flanking 5'-TA-3' dinucleotide prevents excision of an internal eliminated sequence from the Paramecium tetraurelia genome.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Programmed genome rearrangements in ciliates.Cellular and molecular life sciences : CMLS · 2020Review
- Environmentally induced plasticity of programmed DNA elimination boosts somatic variability inGenome research · 2019Article
- Cis-acting signals modulate the efficiency of programmed DNA elimination in Paramecium tetraurelia.Nucleic acids research · 2015Article
- A forward genetic screen reveals essential and non-essential RNAi factors in Paramecium tetraurelia.Nucleic acids research · 2014Article
- Spliced DNA sequences in the Paramecium germline: their properties and evolutionary potential.Genome biology and evolution · 2013Article
- The Paramecium germline genome provides a niche for intragenic parasitic DNA: evolutionary dynamics of internal eliminated sequences.PLoS genetics · 2012Article
- Transposon Invasion of the Paramecium Germline Genome Countered by a Domesticated PiggyBac Transposase and the NHEJ Pathway.International journal of evolutionary biology · 2012Article
- Developmentally programmed DNA splicing in Paramecium reveals short-distance crosstalk between DNA cleavage sites.Nucleic acids research · 2008Article
- Article
- Analysis of Paramecium tetraurelia A-51 surface antigen gene mutants reveals positive-feedback mechanisms for maintenance of expression and temperature-induced activation.Eukaryotic cell · 2005Article
- Processing of double-strand breaks is involved in the precise excision of paramecium internal eliminated sequences.Molecular and cellular biology · 2003Article
- Developmentally regulated chromosome fragmentation linked to imprecise elimination of repeated sequences in paramecia.Eukaryotic cell · 2003Article
- Control of DNA excision efficiency in Paramecium.Nucleic acids research · 2001Article
- Developmentally regulated excision of a 28-base-pair sequence from the Paramecium genome requires flanking DNA.Molecular and cellular biology · 2000Article
- Article
- Timing of developmentally programmed excision and circularization of Paramecium internal eliminated sequences.Molecular and cellular biology · 2000Article
- Developmental timing of programmed DNA elimination inGenome researchArticle
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The germline chromosomes in Paramecium and other ciliated protozoa contain regions of DNA that are excised and eliminated during the development of a new macronuclear genome. Paramecium tetraurelia internal eliminated sequences (IESs) are invariably flanked by a 5'-TA-3' dinucleotide sequence that is part of a larger 8-bp terminal inverted-repeat consensus sequence. Both features, the absolutely conserved 5'-TA-3' and the remaining 6-bp terminal inverted repeat, are shared with the mariner/Tc1 class of transposons. In this article we describe a mutant cell line (AIM-2) defective in excision of a single IES from the coding region of the A51 surface antigen gene. Excision of the 370-bp IES6649 is prevented by a single A to G transition in the invariably conserved 5'-TA-3' dinucleotide. Failure to excise IES6649 also revealed a 29-bp IES located inside IES6649. Additional experiments with the previously isolated AIM-1 mutant, which also contains an internal IES, shows that alternate excision using the wild-type end of IES2591 with an end from the internal IES is extremely rare or nonexistent. These results indicate that IESs are discrete elements whose excision depends upon nucleotides located within the consensus sequence, but also suggest that additional information is required to match one end of an IES with its excision partner.
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