ArticleThe EMBO journal1999
Identification of MINUS, a small polypeptide that functions as a microtubule nucleation suppressor.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Microtubule Dynamics and Neuronal Excitability: Advances on Cytoskeletal Components Implicated in Epileptic Phenomena.Cellular and molecular neurobiology · 2022Review
- Association of Microtubule Dynamics with Chronic Epilepsy.Molecular neurobiology · 2016Article
- GDP-tubulin incorporation into growing microtubules modulates polymer stability.The Journal of biological chemistry · 2010Article
- Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits.The Journal of cell biology · 2000Article
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5 authors.
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Abstract
In eukaryotic cells, tubulin polymerization must be regulated precisely during cell division and differentiation. To identify new mechanisms involved in cellular microtubule formation, we isolated an activity that suppresses microtubule nucleation in vitro. The activity was due to a small acidic polypeptide of 4.7 kDa which we named MINUS (microtubule nucleation suppressor). MINUS inhibited tau- and taxol-mediated microtubule assembly in vitro and was inactivated by dephosphorylation. The protein was purified to homogeneity from cultured neural (PC12) cells and bovine brain. Microinjection of MINUS caused a transient loss of dynamic microtubules in Vero cells. The results suggest that MINUS acts with a novel mechanism on tubulin polymerization, thus regulating microtubule formation in living cells.
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