ArticleThe Journal of physiology1999
Ca2+-independent myosin II phosphorylation and contraction in chicken embryo fibroblasts.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 42 citations in OpenAlex.
- Direct investigation of cell contraction signal networks by light-based perturbation methods.Pflugers Archiv : European journal of physiology · 2023Review
- Transcriptomic analysis of chicken Myozenin 3 regulation reveals its potential role in cell proliferation.PloS one · 2017Article
- Inducible NOS mediates CNP-induced relaxation of intestinal myofibroblasts.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- β-Arrestin regulation of myosin light chain phosphorylation promotes AT1aR-mediated cell contraction and migration.PloS one · 2013Article
- Stellate cell contraction: role, regulation, and potential therapeutic target.Clinics in liver disease · 2008Review
- Sustained contraction and loss of NO production in TGFbeta1-treated endothelial cells.British journal of pharmacology · 2006Article
- Signaling mechanism of thrombin-induced gingival fibroblast-populated collagen gel contraction.British journal of pharmacology · 2006Article
- Rho-kinase-mediated Ca2+-independent contraction in rat embryo fibroblasts.American journal of physiology. Cell physiology · 2004Article
- Article
- Preferential role of intracellular Ca2+ stores in regulation of isometric force in NIH 3T3 fibroblast fibres.The Journal of physiology · 2000Article
- Signal transduction by G-proteins, rho-kinase and protein phosphatase to smooth muscle and non-muscle myosin II.The Journal of physiology · 2000Review
- Myosin and gelsolin cooperate in actin filament severing and actomyosin motor activity.The Journal of biological chemistryArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Non-muscle contraction is widely believed to be mediated through Ca2+-stimulated myosin II regulatory light chain (LC20) phosphorylation, similar to the contractile regulation of smooth muscle. However, this hypothesis lacks conclusive experimental support. 2. By modulating chicken embryo fibroblast cytosolic Ca2+ concentration ([Ca2+]i), we investigated the putative role of [Ca2+]i in fetal bovine serum (FBS)-stimulated LC20 phosphorylation and force development in these cells. 3. Eliminating the FBS-stimulated rise in [Ca2+]i with the Ca2+ chelator BAPTA only partially inhibited FBS-stimulated LC20 phosphorylation and did not significantly alter the magnitude of FBS-stimulated isometric contraction. 4. Ionomycin (1 microM) produced a larger but shorter lasting rise in [Ca2+]i relative to FBS. However, ionomycin only stimulated a small and transient increase in LC20 phosphorylation and did not cause contraction. 5. We conclude that fibroblasts differ from smooth muscle in that LC20 phosphorylation and contraction are predominantly regulated independently of [Ca2+]i.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.