ArticleThe Journal of general physiology1999
Active nuclear import and export is independent of lumenal Ca2+ stores in intact mammalian cells.
Article in The Journal of general physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 53 citations in OpenAlex.
- A cellular atlas of calcineurin signaling.Biochimica et biophysica acta. Molecular cell research · 2023Review
- Changes in nuclear pore numbers control nuclear import and stress response of mouse hearts.Developmental cell · 2022Article
- The Golgi Calcium ATPase Pump Plays an Essential Role in Adeno-associated Virus Trafficking and Transduction.Journal of virology · 2020Article
- Systematic Discovery of Short Linear Motifs Decodes Calcineurin Phosphatase Signaling.Molecular cell · 2020Article
- Recombinant adeno-associated virus utilizes host cell nuclear import machinery to enter the nucleus.Journal of virology · 2014Article
- Intracellular organelles in the saga of Ca2+ homeostasis: different molecules for different purposes?Cellular and molecular life sciences : CMLS · 2012Review
- Transferrin-PEG-PE modified dexamethasone conjugated cationic lipid carrier mediated gene delivery system for tumor-targeted transfection.International journal of nanomedicine · 2012Article
- Calcium regulation of nucleocytoplasmic transport.Protein & cell · 2011Review
- Sodium-calcium exchanger complexed with GM1 ganglioside in nuclear membrane transfers calcium from nucleoplasm to endoplasmic reticulum.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Glucocorticoid receptor phosphorylation differentially affects target gene expression.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Phosphorylation-regulated nucleocytoplasmic trafficking of internalized fibroblast growth factor-1.Molecular biology of the cell · 2005Article
- Real-time imaging of nuclear permeation by EGFP in single intact cells.Biophysical journal · 2003Article
- Nuclear translation: what is the evidence?RNA (New York, N.Y.) · 2003Review
- Regulation of nuclear pore complex conformation by IP(3) receptor activation.Biophysical journal · 2002Article
- Ca2+-dependent nuclear export mediated by calreticulin.Molecular and cellular biology · 2002Article
- Dexamethasone induces the secretion of annexin I in immature lymphoblastic cells by a calcium-dependent mechanism.Molecular and cellular biochemistry · 2002Article
- HuR regulates p21 mRNA stabilization by UV light.Molecular and cellular biology · 2000Article
- Stimulation of the blue light phototropic receptor NPH1 causes a transient increase in cytosolic Ca2+.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Conformational changes of the in situ nuclear pore complex.Biophysical journal · 1999Article
- Regulation of nuclear envelope permeability in cell death and survival.Nucleus (Austin, Tex.)Article
Corrections and comments
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2 authors at 1 institution in 1 country.
Funding
Abstract
The nuclear pore complex (NPC) mediates communication between the cytoplasm and nucleus in eukaryotic cells. Active transport of large polypeptides as well as passive diffusion of smaller (approximately 10 kD) macromolecules through the NPC can be inhibited by depletion of intracellular Ca2+ stores. However, the physiological relevance of this process for the regulation of nucleocytoplasmic trafficking is not yet clear. We expressed green fluorescent protein (GFP)-tagged glucocorticoid receptor (GR) and mitogen-activated protein (MAP) kinase-activated protein kinase 2 (MK2) to study the effect of Ca2+ store depletion on active transport in HM1 cells, a human embryonic kidney cell line stably transfected with the muscarinic M1 receptor. Dexamethasone-induced nuclear import of GR-GFP and anisomycin-induced nuclear export of GFP-MK2 was monitored by confocal microscopy. We found that store depletion by carbachol, thapsigargin or ionomycin had no effect on GR-GFP import, whereas pretreatment with 1,2-bis-(o-aminophenoxy) ethane-N,N,N', N'-tetraacetic acid-acetoxymethyl ester (BAPTA-AM) attenuated import significantly. Export of GFP-MK2 was not influenced by any pretreatment. Moreover, carbachol stimulated GFP-MK2 translocation to the cytoplasm in the absence of anisomycin. These results demonstrate that Ca2+ store depletion in intact HM1 cells is not directly linked to the inhibition of active protein transport through the NPC. The inhibition of GR-GFP import but not GFP-MK2 export by BAPTA-AM presumably involves a depletion-independent mechanism that interferes with components of the nuclear import pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.