ArticleThe Journal of general physiology1999
Radial localization of inositol 1,4,5-trisphosphate-sensitive Ca2+ release sites in Xenopus oocytes resolved by axial confocal linescan imaging.
Article in The Journal of general physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- CaPhysiological reviews · 2024Review
- Spatial-temporal patterning of CaSeminars in cell & developmental biology · 2019Review
- Picomolar sensitivity to inositol trisphosphate in Xenopus oocytes.Cell calcium · 2015Article
- Microscale imaging of cilia-driven fluid flow.Cellular and molecular life sciences : CMLS · 2015Review
- Signal mass and Ca²⁺ kinetics in local calcium events: a modeling study.Journal of molecular modeling · 2012Article
- Mean field strategies induce unrealistic non-linearities in calcium puffs.Frontiers in physiology · 2011Article
- Recording single-channel activity of inositol trisphosphate receptors in intact cells with a microscope, not a patch clamp.The Journal of general physiology · 2010Article
- Article
- Localization and socialization: experimental insights into the functional architecture of IP3 receptors.Chaos (Woodbury, N.Y.) · 2009Review
- cADPR stimulates SERCA activity in Xenopus oocytes.Cell calcium · 2009Article
- Article
- SERCA pump activity is physiologically regulated by presenilin and regulates amyloid beta production.The Journal of cell biology · 2008Article
- Spatiotemporal patterning of IP3-mediated Ca2+ signals in Xenopus oocytes by Ca2+-binding proteins.The Journal of physiology · 2004Article
- Buffer kinetics shape the spatiotemporal patterns of IP3-evoked Ca2+ signals.The Journal of physiology · 2003Article
- Integrated luminal and cytosolic aspects of the calcium release control.Biophysical journal · 2003Article
- Spatial segregation and interaction of calcium signalling mechanisms in rat hippocampal CA1 pyramidal neurons.The Journal of physiology · 2002Article
- Sparks and puffs in oligodendrocyte progenitors: cross talk between ryanodine receptors and inositol trisphosphate receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Xenopus tropicalis oocytes as an advantageous model system for the study of intracellular Ca(2+) signalling.British journal of pharmacology · 2001Article
- From calcium blips to calcium puffs: theoretical analysis of the requirements for interchannel communication.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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2 authors.
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Abstract
The radial localization and properties of elementary calcium release events ("puffs") were studied in Xenopus oocytes using a confocal microscope equipped with a piezoelectric focussing unit to allow rapid (>100 Hz) imaging of calcium signals along a radial line into the cell with a spatial resolution of <0.7 micrometer. Weak photorelease of caged inositol 1,4,5-trisphosphate (InsP3) evoked puffs arising predominantly within a 6-micrometer thick band located within a few micrometers of the cell surface. Approximately 25% of puffs had a restricted radial spread, consistent with calcium release from a single site. Most puffs, however, exhibited a greater radial spread (3.25 micrometer), likely involving recruitment of radially neighboring release sites. Calcium waves evoked by just suprathreshold stimuli exhibited radial calcium distributions consistent with inward diffusion of calcium liberated at puff sites, whereas stronger flashes evoked strong, short-latency signals at depths inward from puff sites, indicating deep InsP3-sensitive stores activated at higher concentrations of InsP3. Immunolocalization of InsP3 receptors showed punctate staining throughout a region corresponding to the localization of puffs and subplasmalemmal endoplasmic reticulum. The radial organization of puff sites a few micrometers inward from the plasma membrane may have important consequences for activation of calcium-dependent ion channels and "capacitative" calcium influx. However, on the macroscopic (hundreds of micrometers) scale of global calcium waves, release can be considered to occur primarily within a thin, essentially two-dimensional subplasmalemmal shell.
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