ArticleGenes & development1999
Transcriptional repression by the Caenorhabditis elegans germ-line protein PIE-1.
Article in Genes & development, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 124 citations in OpenAlex.
- How germ granules promote germ cell fate.Nature reviews. Genetics · 2024Review
- The TOP-2/condensin II axis silences transcription during germline specification in C. elegans.G3 (Bethesda, Md.) · 2024Article
- A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans.Nature communications · 2024Article
- Germ fate determinants protect germ precursor cell division by reducing septin and anillin levels at the cell division plane.Molecular biology of the cell · 2024Article
- Cellular remodeling and JAK inhibition promote zygotic gene expression in the Ciona germline.EMBO reports · 2024Article
- Article
- Germ cell differentiation requires Tdrd7-dependent chromatin and transcriptome reprogramming marked by germ plasm relocalization.Developmental cell · 2021Article
- Article
- PIE-1 Translation in the Germline Lineage Contributes to PIE-1 Asymmetry in the EarlyG3 (Bethesda, Md.) · 2018Article
- Nutrient-DrivenFrontiers in endocrinology · 2018Article
- Article
- A quiet space during rush hour: Quiescence in primordial germ cells.Stem cell research · 2017Article
- Translational Control of Germ Cell Decisions.Results and problems in cell differentiation · 2017Review
- Efficient generation of transgenic reporter strains and analysis of expression patterns in Caenorhabditis elegans using library MosSCI.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Article
- Repression of somatic cell fate in the germline.Cellular and molecular life sciences : CMLS · 2015Review
- Asymmetric transcript discovery by RNA-seq in C. elegans blastomeres identifies neg-1, a gene important for anterior morphogenesis.PLoS genetics · 2015Article
- P-TEFb, the super elongation complex and mediator regulate a subset of non-paused genes during early Drosophila embryo development.PLoS genetics · 2015Article
- The biology of the germ line in echinoderms.Molecular reproduction and development · 2014Review
- RNA polymerase II C-terminal domain: Tethering transcription to transcript and template.Chemical reviews · 2013Review
- Germ cell specification.Advances in experimental medicine and biology · 2013Review
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the early Caenorhabditis elegans embryo, maternally expressed PIE-1 protein is required in germ-line blastomeres to inhibit somatic differentiation, maintain an absence of mRNA transcription, and block phosphorylation of the RNA polymerase II large subunit (Pol II) carboxy-terminal domain (CTD). We have determined that PIE-1 can function as a transcriptional repressor in cell culture assays. By fusing PIE-1 sequences to the yeast GAL4 DNA-binding domain, we have identified a PIE-1 repression domain that appears to inhibit the transcriptional machinery directly. A sequence element that is required for this repressor activity is similar to the Pol II CTD heptapeptide repeat, suggesting that the PIE-1 repression domain might target a protein complex that can bind the CTD. An alteration of this sequence element that blocks repression also impairs the ability of a transgene to rescue a pie-1 mutation, suggesting that this repressor activity may be important for PIE-1 function in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.