ArticleApplied and environmental microbiology1999
Purification and properties of a xylan-binding endoxylanase from Alkaliphilic bacillus sp. strain K-1.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 165 citations in OpenAlex.
- Carbohydrate-binding modules enhance HThe Journal of biological chemistry · 2024Article
- Isolation and characterization of thermostable and alkali-tolerant cellulase from litter endophytic fungus Bartalinia pondoensis.Folia microbiologica · 2022Article
- Efficient biological pretreatment and bioconversion of corn cob by the sequential application of a Bacillus firmus K-1 cellulase-free xylanolytic enzyme and commercial cellulases.Applied microbiology and biotechnology · 2021Article
- Expression, Characterization and Its Deinking Potential of a Thermostable Xylanase FromFrontiers in bioengineering and biotechnology · 2021Article
- A combined physical-chemical and microbiological approach to unveil the fabrication, provenance, and state of conservation of the Kinkarakawa-gami art.Scientific reports · 2020Article
- Insights into Xylan Degradation and Haloalkaline Adaptation through Whole-Genome Analysis ofGenes · 2018Article
- Catalysis of rice straw hydrolysis by the combination of immobilized cellulase from Aspergillus niger on β-cyclodextrin-Fe3O4 nanoparticles and ionic liquid.BioMed research international · 2015Article
- Cloning and Characterizing the Thermophilic and Detergent Stable Cellulase CelMytB from Saccharophagus sp. Myt-1.Indian journal of microbiology · 2014Article
- Purification and Characterization of Haloalkaline, Organic Solvent Stable Xylanase from Newly Isolated Halophilic Bacterium-OKH.International scholarly research notices · 2014Article
- A novel neutral xylanase with high SDS resistance from Volvariella volvacea: characterization and its synergistic hydrolysis of wheat bran with acetyl xylan esterase.Journal of industrial microbiology & biotechnology · 2013Article
- Biochemical and thermodynamic characteristics of thermo-alkali-stable xylanase from a novel polyextremophilic Bacillus halodurans TSEV1.Extremophiles : life under extreme conditions · 2013Article
- Molecular and biochemical characterization of a new alkaline active multidomain xylanase from alkaline wastewater sludge.World journal of microbiology & biotechnology · 2013Article
- Article
- Cloning, purification and characterization of an alkali-stable endoxylanase from thermophilic Geobacillus sp. 71.World journal of microbiology & biotechnology · 2012Article
- Production of alkaliphilic, halotolerent, thermostable cellulase free xylanase by Bacillus halodurans PPKS-2 using agro waste: single step purification and characterization.World journal of microbiology & biotechnology · 2012Article
- Alkaliphilic bacteria: applications in industrial biotechnology.Journal of industrial microbiology & biotechnology · 2011Review
- Production of Xylanase from Arthrobacter sp. MTCC 6915 Using Saw Dust As Substrate under Solid State Fermentation.Enzyme research · 2011Article
- One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2010Article
- Biosynthesis of selenium nanoparticles using Klebsiella pneumoniae and their recovery by a simple sterilization process.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2010Article
- Multimeric hemicellulases facilitate biomass conversion.Applied and environmental microbiology · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An alkaliphilic bacterium, Bacillus sp. strain K-1, produces extracellular xylanolytic enzymes such as xylanases, beta-xylosidase, arabinofuranosidase, and acetyl esterase when grown in xylan medium. One of the extracellular xylanases that is stable in an alkaline state was purified to homogeneity by affinity adsorption-desorption on insoluble xylan. The enzyme bound to insoluble xylan but not to crystalline cellulose. The molecular mass of the purified xylan-binding xylanase was estimated to be approximately 23 kDa. The enzyme was stable at alkaline pHs up to 12. The optimum temperature and optimum pH of the enzyme activity were 60 degrees C and 5.5, respectively. Metal ions such as Fe2+, Ca2+, and Mg2+ greatly increased the xylanase activity, whereas Mn2+ strongly inhibited it. We also demonstrated that the enzyme could hydrolyze the raw lignocellulosic substances effectively. The enzymatic products of xylan hydrolysis were a series of short-chain xylooligosaccharides, indicating that the enzyme was an endoxylanase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.