ArticleApplied and environmental microbiology1999
Molecular analysis of expression of the lantibiotic pep5 immunity phenotype.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 47 citations in OpenAlex.
- Lantibiotic Reductase LtnJ Substrate Selectivity Assessed with a Collection of Nisin Derivatives as Substrates.Applied and environmental microbiology · 2015Article
- Lantibiotic resistance.Microbiology and molecular biology reviews : MMBR · 2015Review
- ApnI, a transmembrane protein responsible for subtilomycin immunity, unveils a novel model for lantibiotic immunity.Applied and environmental microbiology · 2014Article
- Heterologous expression of thuricin CD immunity genes in Listeria monocytogenes.Antimicrobial agents and chemotherapy · 2014Article
- Insights into Lantibiotic Immunity Provided by Bioengineering of LtnI.Antimicrobial agents and chemotherapy · 2012Article
- Processing of as-48ABC RNA in AS-48 enterocin production by Enterococcus faecalis.Journal of bacteriology · 2008Article
- Sec-mediated transport of posttranslationally dehydrated peptides in Lactococcus lactis.Applied and environmental microbiology · 2006Article
- Overproduction of wild-type and bioengineered derivatives of the lantibiotic lacticin 3147.Applied and environmental microbiology · 2006Article
- Localization and functional analysis of PepI, the immunity peptide of Pep5-producing Staphylococcus epidermidis strain 5.Applied and environmental microbiology · 2004Article
- Functional analysis of the gene cluster involved in production of the bacteriocin circularin A by Clostridium beijerinckii ATCC 25752.Applied and environmental microbiology · 2003Article
- Role of tfxE, but not tfxG, in trifolitoxin resistance.Applied and environmental microbiology · 2002Article
- Dual role of GdmH in producer immunity and secretion of the Staphylococcal lantibiotics gallidermin and epidermin.Applied and environmental microbiology · 2001Article
- Biosynthesis of the lantibiotic mersacidin: organization of a type B lantibiotic gene cluster.Applied and environmental microbiology · 2000Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lantibiotic Pep5 is produced by Staphylococcus epidermidis 5. Within its biosynthetic gene cluster, the immunity gene pepI, providing producer self-protection, is localized upstream of the structural gene pepA. Pep5 production and the immunity phenotype have been found to be tightly coupled (M. Reis, M. Eschbach-Bludau, M. I. Iglesias-Wind, T. Kupke, and H.-G. Sahl, Appl. Environ. Microbiol. 60:2876-2883, 1994). To study this phenomenon, we analyzed pepA and pepI transcription and translation and constructed a number of strains containing various fragments of the gene cluster and expressing different levels of immunity. Complementation of a pepA-expressing strain with pepI in trans did not result in phenotypic immunity or production of PepI. On the other hand, neither pepA nor its product was found to be involved in immunity, since suppression of the translation of the pepA mRNA by mutation of the ATG start codon did not reduce the level of immunity. Moreover, homologous and heterologous expression of pepI from a xylose-inducible promoter resulted in significant Pep5 insensitivity. Most important for expression of the immunity phenotype was the stability of pepI transcripts, which in the wild-type strain, is achieved by an inverted repeat with a free energy of -56.9 kJ/mol, localized downstream of pepA. We performed site-directed mutagenesis to study the functional role of PepI and constructed F13D PepI, I17R PepI, and PepI 1-65; all mutants showed reduced levels of immunity. Western blot analysis indicated that F13D PepI and PepI 1-65 were not produced correctly or were partially degraded, while I17R PepI apparently was less efficient in providing self-protection than the wild-type PepI.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.