ArticleApplied and environmental microbiology1999
Attachment of the yeast Rhodosporidium toruloides is mediated by adhesives localized at sites of bud cell development.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- Surface-Engineered Filters for Wettability-Driven Collection of Airborne Fungal Spores.Global challenges (Hoboken, NJ) · 2026Article
- A membrane-free spot-plating protocol for Agrobacterium-mediated transformation of diverse yeasts.PloS one · 2026Article
- Acidification by nitrogen metabolism triggers extracellular biopolymer production in an oleaginous yeast.Applied and environmental microbiology · 2025Article
- Review
- Potential of Rhodosporidium toruloides for Fatty Acids Production Using Lignocellulose Biomass.Applied biochemistry and biotechnology · 2024Review
- Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock.Microbial cell factories · 2024Article
- Secretomic Insights into the Pathophysiology ofPathogens (Basel, Switzerland) · 2022Article
- Oleaginous yeastFrontiers in molecular biosciences · 2022Article
- Potassium and Sodium Salt Stress Characterization in the Yeasts Saccharomyces cerevisiae, Kluyveromyces marxianus, andApplied and environmental microbiology · 2021Article
- Rhodosporidium toruloides - A potential red yeast chassis for lipids and beyond.FEMS yeast research · 2020Review
- Article
- Four Inducible Promoters for Controlled Gene Expression in the Oleaginous YeastFrontiers in microbiology · 2016Article
- Attachment Capability of Antagonistic Yeast Rhodotorula glutinis to Botrytis cinerea Contributes to Biocontrol Efficacy.Frontiers in microbiology · 2016Article
- Isolated microbial single cells and resulting micropopulations grow faster in controlled environments.Applied and environmental microbiology · 2012Article
- The O-mannosyltransferase PMT4 is essential for normal appressorium formation and penetration in Ustilago maydis.The Plant cell · 2009Article
- Oribatid mites as potential vectors for soil microfungi: study of mite-associated fungal species.Microbial ecology · 2005Article
- Growth of Rhodosporidium toruloides strain DBVPG 6662 on dibenzothiophene crystals and orimulsion.Applied and environmental microbiology · 2003Article
- Localized, positive charge mediates adhesion of rhodosporidium toruloides to barley leaves and polystyreneApplied and environmental microbiology · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The basidiomycetous yeast Rhodosporidium toruloides (anamorph, Rhodotorula glutinis) is a common phylloplane epiphyte with biocontrol potential. To understand how R. toruloides adheres to plant surfaces, we obtained nonadherent fungal mutants after chemical mutagenesis with methane-sulfonic acid ethyl ester. Sixteen attachment-minus (Att-) mutants were identified by three methods: (i) screening capsule-minus colonies for loss of adhesive ability; (ii) enrichment for mutants unable to attach to polystyrene; and (iii) selection for reduced fluorescence of fluorescein isothiocyanate-concanavalin A (Con A)-stained cells by fluorescence-activated cell sorting. None of the 16 mutants attached to polystyrene or barley leaves. The lectin Con A eliminated adhesion in all of the wild-type isolates tested. Hapten competition assays indicated that Con A bound to mannose residues on the cell surface. Adhesion of wild-type R. toruloides was transient; nonadhesive cells subsequently became adhesive, with bud development. All Att- mutants and nonattaching wild-type cells lacked polar regions that stained intensely with fluorescein isothiocyanate-Con A and India ink. Lectin, enzyme, and chemical treatments showed that the polar regions consisted of alkali-soluble materials, including mannose residues. Tunicamycin treatment reduced wild-type adhesion, indicating that the mannose residues could be associated with glycoproteins. We concluded that compounds, including mannose residues, that are localized at sites of bud development mediate adhesion of R. toruloides to both polystyrene and barley leaf surfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.