ArticleThe Journal of cell biology1999
The small GTP-binding protein rho regulates cortical activities in cultured cells during division.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
57 citing papers in PubMed, 123 citations in OpenAlex.
- Septins regulate border cell surface geometry, shape, and motility downstream of Rho in Drosophila.Developmental cell · 2023Article
- Identification of a small RhoA GTPase inhibitor effective in fission yeast and human cells.Open biology · 2023Article
- Role of Small GTPase RhoA in DNA Damage Response.Biomolecules · 2021Review
- Crosslinking activity of non-muscle myosin II is not sufficient for embryonic cytokinesis inDevelopment (Cambridge, England) · 2019Article
- CYK-4 regulates Rac, but not Rho, during cytokinesis.Molecular biology of the cell · 2017Article
- Positioning the cleavage furrow: All you need is Rho.The Journal of cell biology · 2016Article
- Foci of cyclin A2 interact with actin and RhoA in mitosis.Scientific reports · 2016Article
- Cytokinesis involves a nontranscriptional function of the Hippo pathway effector YAP.Science signaling · 2016Article
- Mitochondria-cytoskeleton associations in mammalian cytokinesis.Cell division · 2016Article
- MgcRacGAP restricts active RhoA at the cytokinetic furrow and both RhoA and Rac1 at cell-cell junctions in epithelial cells.Molecular biology of the cell · 2015Article
- Cytokinetic Failure-induced Tetraploidy Develops into Aneuploidy, Triggering Skin Aging in Phosphovimentin-deficient Mice.The Journal of biological chemistry · 2015Article
- Controlling the switches: Rho GTPase regulation during animal cell mitosis.Cellular signalling · 2014Review
- Small GTPase RhoA regulates cytoskeleton dynamics during porcine oocyte maturation and early embryo development.Cell cycle (Georgetown, Tex.) · 2014Article
- Quantitative analysis of cytokinesis in situ during C. elegans postembryonic development.PloS one · 2014Article
- Defect of mitotic vimentin phosphorylation causes microophthalmia and cataract via aneuploidy and senescence in lens epithelial cells.The Journal of biological chemistry · 2013Article
- Serum regulation of Id1 expression by a BMP pathway and BMP responsive element.Biochimica et biophysica acta · 2013Article
- Interphase cytofission maintains genomic integrity of human cells after failed cytokinesis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis.The Journal of cell biology · 2013Article
- Rho GTPases in animal cell cytokinesis: an occupation by the one percent.Cytoskeleton (Hoboken, N.J.) · 2012Review
- Cytokinesis-based constraints on polarized cell growth in fission yeast.PLoS genetics · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
We have investigated the role of the small GTP-binding protein Rho in cytokinesis by microinjecting an inhibitor, C3 ribosyltransferase, into cultured cells. Microinjection of C3 into prometaphase or metaphase normal rat kidney epithelial cells induced immediate and global cortical movement of actin toward the metaphase plate, without an apparent effect on the mitotic spindle. During anaphase, concentrated cortical actin filaments migrated with separating chromosomes, leaving no apparent concentration of actin filaments along the equator. Myosin II in injected epithelial cells showed a diffuse distribution throughout cell division. All treated, well-adherent cells underwent cleavage-like activities and most of them divided successfully. However, cytokinesis became abnormal, generating irregular ingressions and ectopic cleavage sites even when mitosis was blocked with nocodazole. The effects of C3 appeared to be dependent on cell adhesion; less adherent 3T3 fibroblasts exhibited irregular cortical ingression only when cells started to increase attachment during respreading, but managed to complete cytokinesis. Poorly adherent HeLa cells showed neither ectopic cleavage nor completion of cytokinesis. Our results indicate that Rho does not simply activate actin-myosin II interactions during cytokinesis, but regulates the spatial pattern of cortical activities and completion of cytokinesis possibly through modulating the mechanical strength of the cortex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.