ArticleThe Journal of cell biology1999
Temporal differences in the appearance of NEP-B78 and an LBR-like protein during Xenopus nuclear envelope reassembly reflect the ordered recruitment of functionally discrete vesicle types.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 70 citations in OpenAlex.
- The Biology of the Nuclear Envelope and Its Implications in Cancer Biology.International journal of molecular sciences · 2019Review
- The different function of single phosphorylation sites of Drosophila melanogaster lamin Dm and lamin C.PloS one · 2012Article
- Temporal control of nuclear envelope assembly by phosphorylation of lamin B receptor.Molecular biology of the cell · 2011Article
- Differential nuclear envelope assembly at the end of mitosis in suspension-cultured Apium graveolens cells.Chromosoma · 2010Article
- Transmembrane protein-free membranes fuse into xenopus nuclear envelope and promote assembly of functional pores.The Journal of biological chemistry · 2009Article
- Reticulon 4a/NogoA locates to regions of high membrane curvature and may have a role in nuclear envelope growth.Journal of structural biology · 2007Article
- A novel role for the nuclear membrane protein emerin in association of the centrosome to the outer nuclear membrane.The Journal of cell biology · 2007Article
- Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules.Molecular biology of the cell · 2006Article
- Importin beta negatively regulates nuclear membrane fusion and nuclear pore complex assembly.Molecular biology of the cell · 2003Article
- A role for nuclear lamins in nuclear envelope assembly.The Journal of cell biology · 2001Article
- Nuclear lamins A and B1: different pathways of assembly during nuclear envelope formation in living cells.The Journal of cell biology · 2000Article
- Incorporation of the nuclear pore basket protein nup153 into nuclear pore structures is dependent upon lamina assembly: evidence from cell-free extracts of Xenopus eggs.The EMBO journal · 2000Article
- In vitro formation of the endoplasmic reticulum occurs independently of microtubules by a controlled fusion reaction.The Journal of cell biology · 2000Article
- The nuclear envelope as a chromatin organizer.Nucleus (Austin, Tex.)Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, we have used novel mAbs against two proteins of the endoplasmic reticulum and outer nuclear membrane, termed NEP-B78 and p65, in addition to a polyclonal antibody against the inner nuclear membrane protein LBR (lamin B receptor), to study the order and dynamics of NE reassembly in the Xenopus cell-free system. Using these reagents, we demonstrate differences in the timing of recruitment of their cognate membrane proteins to the surface of decondensing chromatin in both the cell-free system and XLK-2 cells. We show unequivocally that, in the cell-free system, two functionally and biochemically distinct vesicle types are necessary for NE assembly. We find that the process of distinct vesicle recruitment to chromatin is an ordered one and that NEP-B78 defines a vesicle population involved in the earliest events of reassembly in this system. Finally, we present evidence that NEP-B78 may be required for the targeting of these vesicles to the surface of decondensing chromatin in this system. The results have important implications for the understanding of the mechanisms of nuclear envelope disassembly and reassembly during mitosis and for the development of systems to identify novel molecules that control these processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.