ArticleJournal of bacteriology1999
The presence of glutamate dehydrogenase is a selective advantage for the Cyanobacterium synechocystis sp. strain PCC 6803 under nonexponential growth conditions.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- NativeInternational journal of molecular sciences · 2025Article
- The adc1 knockout with proC overexpression in Synechocystis sp. PCC 6803 induces a diversion of acetyl-CoA to produce more polyhydroxybutyrate.Biotechnology for biofuels and bioproducts · 2024Article
- Basin-scale biogeography of marine phytoplankton reflects cellular-scale optimization of metabolism and physiology.Science advances · 2022Article
- Nitrogen Availability Affects the Metabolic Profile in Cyanobacteria.Metabolites · 2021Article
- Pressurized Liquid Extraction of a Phycocyanobilin Chromophore and Its Reconstitution with a Cyanobacteriochrome Photosensor for Efficient Isotopic Labeling.Plant & cell physiology · 2021Article
- Essentiality of the glnA gene in Haloferax mediterranei: gene conversion and transcriptional analysis.Extremophiles : life under extreme conditions · 2020Article
- The Distinctive Regulation of Cyanobacterial Glutamine Synthetase.Life (Basel, Switzerland) · 2018Review
- A review of computational tools for design and reconstruction of metabolic pathways.Synthetic and systems biotechnology · 2017Review
- Computational metabolic engineering strategies for growth-coupled biofuel production byMetabolic engineering communications · 2016Article
- Temporal Gene Expression of the Cyanobacterium Arthrospira in Response to Gamma Rays.PloS one · 2015Article
- Cell-specific gene expression in Anabaena variabilis grown phototrophically, mixotrophically, and heterotrophically.BMC genomics · 2013Article
- Patterns and implications of gene gain and loss in the evolution of Prochlorococcus.PLoS genetics · 2007Article
- Ammonium assimilation in cyanobacteria.Photosynthesis research · 2005Review
- Streamlined regulation and gene loss as adaptive mechanisms in Prochlorococcus for optimized nitrogen utilization in oligotrophic environments.Microbiology and molecular biology reviews : MMBR · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The unicellular cyanobacterium Synechocystis sp. strain PCC 6803 has two putative pathways for ammonium assimilation: the glutamine synthetase-glutamate synthase cycle, which is the main one and is finely regulated by the nitrogen source; and a high NADP-dependent glutamate dehydrogenase activity (NADP-GDH) whose contribution to glutamate synthesis is uncertain. To investigate the role of the latter, we used two engineered mutants, one lacking and another overproducing NADP-GDH. No major disturbances in the regulation of nitrogen-assimilating enzymes or in amino acids pools were detected in the null mutant, but phycobiline content, a sensitive indicator of the nutritional state of cyanobacterial cells, was significantly reduced, indicating that NADP-GDH plays an auxiliary role in ammonium assimilation. This effect was already prominent in the initial phase of growth, although differences in growth rate between the wild type and the mutants were observed at this stage only at low light intensities. However, the null mutant was unable to sustain growth at the late stage of the culture at the point when the wild type showed the maximum NADP-GDH activity, and died faster in ammonium-containing medium. Overexpression of NADP-GDH improved culture proliferation under moderate ammonium concentrations. Competition experiments between the wild type and the null mutant confirmed that the presence of NADP-GDH confers a selective advantage to Synechocystis sp. strain PCC 6803 in late stages of growth.
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