ArticleAnalytical biochemistry1999
Glucose sensor for low-cost lifetime-based sensing using a genetically engineered protein.
Article in Analytical biochemistry, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed, 188 citations in OpenAlex.
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Unpacking the packaged optical fiber bio-sensors: understanding the obstacle for biomedical application.Frontiers in bioengineering and biotechnology · 2024Review
- Photophysical Properties of BADAN Revealed in the Study of GGBP Structural Transitions.International journal of molecular sciences · 2021Article
- Noninvasive Glucose Monitoring with a Contact Lens and Smartphone.Sensors (Basel, Switzerland) · 2018Article
- Osmolyte-Like Stabilizing Effects of Low GdnHCl Concentrations on d-Glucose/d-Galactose-Binding Protein.International journal of molecular sciences · 2017Article
- The role of water molecules in stereoselectivity of glucose/galactose-binding protein.Scientific reports · 2016Article
- Studies of conformational changes of an arginine-binding protein from Thermotoga maritima in the presence and absence of ligand via molecular dynamics simulations with the coarse-grained UNRES force field.Journal of molecular modeling · 2015Article
- Tryptophan residue of the D-galactose/D-glucose-binding protein from E. Coli localized in its active center does not contribute to the change in intrinsic fluorescence upon glucose binding.Journal of fluorescence · 2015Article
- Passive Diffusion of Transdermal Glucose: Noninvasive Glucose Sensing Using a Fluorescent Glucose Binding Protein.Journal of diabetes science and technology · 2014Article
- Article
- Optimization of a Concanavalin A-based glucose sensor using fluorescence anisotropy.Analytical chemistry · 2013Article
- Fluorescence intensity- and lifetime-based glucose sensing using glucose/galactose-binding protein.Journal of diabetes science and technology · 2013Review
- Detection of trace glucose on the surface of a semipermeable membrane using a fluorescently labeled glucose-binding protein: a promising approach to noninvasive glucose monitoring.Journal of diabetes science and technology · 2013Article
- Harnessing a nanostructured fluorescence energy transfer sensor for quick detection of extremely small amounts of glucose.Journal of diabetes science and technology · 2013Article
- Crystallization and preliminary X-ray crystallographic analysis of ligand-free and arginine-bound forms of Thermotoga maritima arginine-binding protein.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Thermal motions of the E. coli glucose-galactose binding protein studied using well-sampled, semi-atomistic simulations.Current topics in medicinal chemistry · 2011Article
- Engineering periplasmic ligand binding proteins as glucose nanosensors.Nano reviews · 2011Article
- A luminescence lifetime assisted ratiometric fluorimeter for biological applications.The Review of scientific instruments · 2009Article
- Dissolved core alginate microspheres as "smart-tattoo" glucose sensors.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2009Article
- Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity.Biophysical journal · 2008Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
We describe a glucose sensor based on a mutant glucose/galactose binding protein (GGBP) and phase-modulation fluorometry. The GGBP from Escherichia coli was mutated to contain a single cysteine residue at position 26. When labeled with a sulfhydryl-reactive probe 2-(4'-iodoacetamidoanilino)naphthalene-6-sulfonic acid, the labeled protein displayed a twofold decrease in intensity in response to glucose, with a dissociation constant near 1 microM glucose. The ANS-labeled protein displayed only a modest change in lifetime, precluding lifetime-based sensing of glucose. A modulation sensor was created by combining ANS26-GGBP with a long-lifetime ruthenium (Ru) metal-ligand complex on the surface of the cuvette. Binding of glucose changed the relative intensity of ANS26-GGBP and the Ru complex, resulting in a dramatic change in modulation at a low frequency of 2.1 MHz. Modulation measurements at 2.1 MHz were shown to accurately determine the glucose concentration. These results suggest an approach to glucose sensing with simple devices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.