ArticleRNA (New York, N.Y.)1999
Sequence specificity of in vivo reverse splicing of the Tetrahymena group I intron.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 50 citations in OpenAlex.
- A Phylogenetic Approach to Structural Variation in Organization of Nuclear Group I Introns and Their Ribozymes.Non-coding RNA · 2021Article
- Intra-specific comparison of mitochondrial genomes reveals host gene fragment exchange via intron mobility in Tremella fuciformis.BMC genomics · 2020Article
- The diversity of mtDNA rns introns among strains of Ophiostoma piliferum, Ophiostoma pluriannulatum and related species.SpringerPlus · 2016Article
- Horizontal transfer and gene conversion as an important driving force in shaping the landscape of mitochondrial introns.G3 (Bethesda, Md.) · 2014Article
- Nuclear group I introns in self-splicing and beyond.Mobile DNA · 2013Article
- Genomes of the T4-related bacteriophages as windows on microbial genome evolution.Virology journal · 2010Review
- Group I introns and inteins: disparate origins but convergent parasitic strategies.Journal of bacteriology · 2009Review
- Evolution of Pleopsidium (lichenized Ascomycota) S943 group I introns and the phylogeography of an intron-encoded putative homing endonuclease.Journal of molecular evolution · 2007Article
- Phylogenetic analyses suggest reverse splicing spread of group I introns in fungal ribosomal DNA.BMC evolutionary biology · 2005Article
- Site-specific reverse splicing of a HEG-containing group I intron in ribosomal RNA.Nucleic acids research · 2005Article
- Molecular recognition properties of IGS-mediated reactions catalyzed by a Pneumocystis carinii group I intron.Nucleic acids research · 2003Article
- The tmRNA Website: invasion by an intron.Nucleic acids research · 2002Article
- Barriers to intron promiscuity in bacteria.Journal of bacteriology · 2000Review
- Evaluating group I intron catalytic efficiency in mammalian cells.Molecular and cellular biology · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse splicing of group I introns is proposed to be a mechanism by which intron sequences are transferred to new genes. Integration of the Tetrahymena intron into the Escherichia coli 23S rRNA via reverse splicing depends on base pairing between the guide sequence of the intron and the target site. To investigate the substrate specificity of reverse splicing, the wild-type and 18 mutant introns with different guide sequences were expressed in E. coli. Amplification of intron-rRNA junctions by RT-PCR revealed partial reverse splicing at 69 sites and complete integration at one novel site in the 23S rRNA. Reverse splicing was not observed at some potential target sites, whereas other regions of the 23S rRNA were more reactive than expected. The results indicate that the frequency of reverse splicing is modulated by the structure of the rRNA. The intron is spliced 10-fold less efficiently in E. coli from a novel integration site (U2074) in domain V of the 23S rRNA than from a site homologous to the natural splice junction of the Tetrahymena 26S rRNA, suggesting that the forward reaction is less favored at this site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.