ArticleThe American journal of pathology1999
Detection of clonal T-cell receptor gamma gene rearrangements in paraffin-embedded tissue by polymerase chain reaction and nonradioactive single-strand conformational polymorphism analysis.
Article in The American journal of pathology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 74 citations in OpenAlex.
- Sal-Like Protein 4 Transcription Factor: A Significant Diagnostic Biomarker Involved in Childhood ALL Resistance and Relapse.Cancer management and research · 2020Article
- Molecular Testing of Lymphoproliferative Disorders: Current Status and Perspectives.Journal of pathology and translational medicine · 2017Review
- First report on familial hemophagocytic lymphohistiocytosis with an abnormal immunophenotype and T cell monoclonality in Korea.Annals of laboratory medicine · 2015Article
- EBV-Positive T/NK-Cell Lymphoproliferative Disease of Childhood.Korean journal of pathology · 2013Article
- Comparison of BIOMED-2 versus laboratory-developed polymerase chain reaction assays for detecting T-cell receptor-gamma gene rearrangements.The Journal of molecular diagnostics : JMD · 2010Article
- Primary T-cell lymphoma of the thyroid associated with Hashimoto's thyroiditis, histologically mimicking MALT-lymphoma.Journal of Korean medical science · 2010Article
- Mycosis fungoides as an ichthyosiform eruption.Annals of dermatology · 2009Article
- Comparative investigations of T cell receptor gamma gene rearrangements in frozen and formalin-fixed paraffin wax-embedded tissues by capillary electrophoresis.Journal of clinical pathology · 2006Article
- Expression of CD56 antigen in Langerhans cell histiocytosis associated with T-lymphoblastic lymphoma in a same lymph node.Virchows Archiv : an international journal of pathology · 2006Article
- Hepatosplenic gammadelta T-cell lymphoma.World journal of gastroenterology · 2005Article
- CD4+CD56+ lineage negative hematopoietic neoplasm: so called blastic NK cell lymphoma.Journal of Korean medical science · 2005Article
- Rapid detection of clonal T-cell receptor-beta gene rearrangements in T-Cell lymphomas using the LightCycler-polymerase chain reaction with DNA melting curve analysis.The Journal of molecular diagnostics : JMD · 2005Article
- Investigation of T-cell receptor-gamma gene rearrangement in gastrointestinal lymphomas by PCR-SSCP analysis.World journal of gastroenterology · 2004Article
- Effectiveness of capillary electrophoresis using fluorescent-labeled primers in detecting T-cell receptor gamma gene rearrangements.The Journal of molecular diagnostics : JMD · 2002Article
- Quantitative assessment of minimal residual disease in childhood lymphoid malignancies using an allele-specific oligonucleotide real-time quantitative polymerase chain reaction.International journal of hematology · 2002Article
- Article
- Restricted killer cell immunoglobulin-like receptor repertoire without T-cell receptor gamma rearrangement supports a true natural killer-cell lineage in a subset of sinonasal lymphomas.The American journal of pathology · 2001Article
- Advances in molecular hematopathology: T-cell receptor gamma and bcl-2 genes.The American journal of pathology · 1999Review
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6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diagnosis of T-cell lymphoproliferative disorders, which frequently involve the skin and other extranodal sites, is often problematic because of the difficulty in establishing clonality in paraffin-embedded tissue. To this end, we developed a simple, nonradioactive method to detect T-cell receptor gamma (TCR-gamma) gene rearrangements by polymerase chain reaction single-strand conformational polymorphism (PCR-SSCP) in paraffin-embedded tissue. Jurkat and HSB-2 cell lines and peripheral blood samples from normal individuals were used as monoclonal and polyclonal controls, respectively. DNA was extracted from 24 biopsies of T-cell lymphomas, 12 biopsies of reactive lymphoid infiltrates, and 2 biopsies of primary cutaneous large B-cell lymphomas. Vgamma1-8, Vgamma9, Vgamma10, Vgamma11, and Jgamma1/Jgamma2 consensus primers were used for TCR-gamma gene rearrangement amplification and PCR products were analyzed by nonradioactive SSCP. Monoclonal controls yielded a well-defined banded pattern, whereas all polyclonal T-cell controls showed a reproducible pattern of smears. We detected monoclonality in 20/21 (95%) T-cell lymphoma cases, whereas no dominant T-cell clones were found in any of the reactive lymphoid infiltrates or B-cell lymphomas. Sensitivity of 1-5% was demonstrated by serially diluting Jurkat cells in mononuclear blood cells from normal individuals. We conclude that nonradioactive PCR-SSCP for TCR-gamma gene rearrangement analysis is a useful adjunct to routine histological and immunophenotypic methods in the diagnosis of T-cell lymphoproliferative disorders in paraffin-embedded tissue.
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