ArticleInfection and immunity1999
Regulation of ExoS production and secretion by Pseudomonas aeruginosa in response to tissue culture conditions.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed, 148 citations in OpenAlex.
- TypA Interacts with ExsA to Suppress Type III Secretion System inMicroorganisms · 2026Article
- Defining the role ofJournal of bacteriology · 2026Article
- The quorum sensing regulator RhlR positively controls the expression of the type III secretion system in Pseudomonas aeruginosa PAO1.PloS one · 2024Article
- Type III Secretion inMicrobiology and molecular biology reviews : MMBR · 2023Review
- tRNA modification enzyme MiaB connects environmental cues to activation of Pseudomonas aeruginosa type III secretion system.PLoS pathogens · 2022Article
- Maintenance of tRNA and elongation factors supports T3SS proteins translational elongations in pathogenic bacteria during nutrient starvation.Cell & bioscience · 2022Article
- New roles for glutathione: Modulators of bacterial virulence and pathogenesis.Redox biology · 2021Article
- Calcium regulates the mycophagous ability of Burkholderia gladioli strain NGJ1 in a type III secretion system-dependent manner.BMC microbiology · 2020Article
- Calcium Regulation of Bacterial Virulence.Advances in experimental medicine and biology · 2020Review
- Early Cytokine Induction UponFrontiers in immunology · 2020Article
- Pseudomonas aeruginosa ExsA Regulates a Metalloprotease, ImpA, That Inhibits Phagocytosis of Macrophages.Infection and immunity · 2019Article
- RNase E Promotes Expression of Type III Secretion System Genes in Pseudomonas aeruginosa.Journal of bacteriology · 2019Article
- Tanshinones: First-in-Class Inhibitors of the Biogenesis of the Type 3 Secretion System Needle ofACS central science · 2019Article
- Fitting Pieces into the Puzzle ofJournal of bacteriology · 2019Review
- A Unique ATPase, ArtR (PA4595), Represses the Type III Secretion System inFrontiers in microbiology · 2019Article
- The Impact of ExoS onmBio · 2018Article
- Epithelial cell lysates induce ExoS expression and secretion by Pseudomonas aeruginosa.FEMS microbiology letters · 2018Article
- Inhibition of Pseudomonas aeruginosa ExsA DNA-Binding Activity by N-Hydroxybenzimidazoles.Antimicrobial agents and chemotherapy · 2016Article
- Glutamate Limitation, BvgAS Activation, and (p)ppGpp Regulate the Expression of the Bordetella pertussis Type 3 Secretion System.Journal of bacteriology · 2016Article
- ExsE Is a Negative Regulator for T3SS Gene Expression inFrontiers in cellular and infection microbiology · 2016Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
This study was initiated to characterize the regulation and secretion of ExoS by Pseudomonas aeruginosa during contact with eukaryotic cells. The production of ExoS was monitored by a sensitive ADP-ribosyltransferase activity assay, and specific activities were calculated for supernatant and cell-associated fractions. Time course analysis indicated that ExoS was produced after a lag period, suggesting that induction of the regulon is necessary for the expression of detectable amounts of enzyme activity. Under tissue culture growth conditions, ExoS was induced when P. aeruginosa was in contact with Chinese hamster ovary (CHO) cells or after growth in tissue culture medium with serum. The serum induction of ExoS appeared to result in generalized type III secretion, while induction by contact with CHO cells appeared to result in polarized type III secretion. Mutants in the type III secretory system that express a null phenotype for ExoS production in bacteriological medium produced but did not secrete the enzyme when P. aeruginosa was grown under inducing conditions in tissue culture medium. These results suggest that both induction and secretion of ExoS may differ when the bacteria are exposed to different growth environments. The putative type III translocation proteins and secretion apparatus of P. aeruginosa were required for translocation of bacterial factors that mediate changes in CHO cell morphology during infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.