ArticleInfection and immunity1999
The URA5 gene is necessary for histoplasma capsulatum growth during infection of mouse and human cells.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Tools for genetic manipulation of the endemic fungal pathogen,bioRxiv : the preprint server for biology · 2025Article
- Article
- Article
- Genotypic diversity, virulence, and molecular genetic tools inMicrobiology and molecular biology reviews : MMBR · 2024Review
- Resistance profiling ofEmerging microbes & infections · 2022Article
- CRISPR-Cas9-Based Mutagenesis of the Mucormycosis-Causing FungusInternational journal of molecular sciences · 2020Article
- Applications of Invertebrate Animal Models to Dimorphic Fungal Infections.Journal of fungi (Basel, Switzerland) · 2018Review
- F901318 represents a novel class of antifungal drug that inhibits dihydroorotate dehydrogenase.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Revisiting old friends: Developments in understanding Histoplasma capsulatum pathogenesis.Journal of microbiology (Seoul, Korea) · 2016Review
- Histoplasma capsulatum surmounts obstacles to intracellular pathogenesis.The FEBS journal · 2016Review
- Histoplasma capsulatum depends on de novo vitamin biosynthesis for intraphagosomal proliferation.Infection and immunity · 2014Article
- Secreted dipeptidyl peptidase IV activity in the dimorphic fungal pathogen Histoplasma capsulatum.Infection and immunity · 2009Article
- RNA interference-mediated silencing of the YPS3 gene of Histoplasma capsulatum reveals virulence defects.Infection and immunity · 2007Article
- Expression and interstrain variability of the YPS3 gene of Histoplasma capsulatum.Eukaryotic cell · 2007Article
- Sequence elements necessary for transcriptional activation of BAD1 in the yeast phase of Blastomyces dermatitidis.Eukaryotic cell · 2004Article
- Fungal virulence studies come of age.Genome biology · 2001Review
- Histoplasma capsulatum strain variation in both H antigen production and beta-glucosidase activity and overexpression of HAG1 from a telomeric linear plasmid.Infection and immunity · 1999Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The Histoplasma capsulatum URA5 gene, which has recently been cloned and disrupted by allelic replacement, encodes orotidine-5'-monophosphate pyrophosphorylase. Inactivation of URA5 by either targeted or UV mutagenesis results in disruption of the pyrimidine biosynthetic pathway and uracil auxotrophy. We examined the effect of uracil auxotrophy due to a ura5 mutation on H. capsulatum virulence in both cell culture and whole-animal models. Uracil auxotrophs of two H. capsulatum restriction fragment length polymorphism classes were found to be avirulent in cultured murine and human cells, as well as in mice. Moreover, virulence could be restored either by supplying a functional URA5 gene in trans or by supplying exogenous uracil during infection in vitro. These experiments demonstrate that the pyrimidine biosynthetic pathway is essential for H. capsulatum growth and virulence.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.