ArticleThe Biochemical journal1999
Epidermal growth factor receptor activation is localized within low-buoyant density, non-caveolar membrane domains.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 145 citations in OpenAlex.
- New insights into lipid metabolism and prostate cancer (Review).International journal of oncology · 2023Review
- Afterword (Editorial).Glycoconjugate journal · 2023Article
- PI(4,5)PAdvances in experimental medicine and biology · 2023Article
- Rafting on the Plasma Membrane: Lipid Rafts in Signaling and Disease.Advances in experimental medicine and biology · 2023Review
- Ganglioside GM1 promotes contact inhibition of growth by regulating the localization of epidermal growth factor receptor from glycosphingolipid-enriched microdomain to caveolae.Cell proliferation · 2019Article
- The endogenous subcellular localisations of the long chain fatty acid-activating enzymes ACSL3 and ACSL4 in sarcoma and breast cancer cells.Molecular and cellular biochemistry · 2018Article
- The Epidermal Growth Factor Receptor Forms Location-Dependent Complexes in Resting Cells.Biophysical journal · 2016Article
- Article
- Caveolin-1 is required for TGF-β-induced transactivation of the EGF receptor pathway in hepatocytes through the activation of the metalloprotease TACE/ADAM17.Cell death & disease · 2014Article
- RhoA localization with caveolin-1 regulates vascular contractions to serotonin.American journal of physiology. Regulatory, integrative and comparative physiology · 2012Article
- A novel biotinylated lipid raft reporter for electron microscopic imaging of plasma membrane microdomains.Journal of lipid research · 2012Article
- Confidence intervals for concentration and brightness from fluorescence fluctuation measurements.Biophysical journal · 2012Article
- Flotillin-1/reggie-2 protein plays dual role in activation of receptor-tyrosine kinase/mitogen-activated protein kinase signaling.The Journal of biological chemistry · 2012Article
- CDP-diacylglycerol phospholipid synthesis in detergent-soluble, non-raft, membrane microdomains of the endoplasmic reticulum.Journal of lipid research · 2011Article
- Detergent-free isolation and characterization of cholesterol-rich membrane domains from trans-Golgi network vesicles.Journal of lipid research · 2011Article
- Elastin peptides signaling relies on neuraminidase-1-dependent lactosylceramide generation.PloS one · 2010Article
- The epidermal growth factor receptor (EGFR) promotes uptake of influenza A viruses (IAV) into host cells.PLoS pathogens · 2010Article
- Relationship between phosphatidylinositol 4-phosphate synthesis, membrane organization, and lateral diffusion of PI4KIIalpha at the trans-Golgi network.Journal of lipid research · 2010Article
- Filamin A modulates kinase activation and intracellular trafficking of epidermal growth factor receptors in human melanoma cells.Endocrinology · 2009Article
- Palmitoylation controls the catalytic activity and subcellular distribution of phosphatidylinositol 4-kinase II{alpha}.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increasing evidence for the organization of cell-surface proteins and lipids into different detergent-insoluble rafts led us to investigate epidermal growth factor (EGF) receptor activation in the plasma membranes of A431 carcinoma cells, using a combination of cell fractionation and immunoprecipitation techniques. Density-gradient centrifugation of sodium carbonate cell extracts revealed that the vast majority of both stimulated and unstimulated EGF receptors were concentrated in a caveolin-rich light membrane (CLM) fraction, with the biochemical characteristics of detergent-insoluble glycolipid-rich domains (DIGs). However, ultrastructural analysis of the CLM fraction revealed that it contained a heterogeneous collection of vesicles, some with sizes greater than that expected for individual caveolae. Experiments with detergent-solubilized cells and isolated CLMs indicated that, in contrast with caveolin, EGF receptors were unlikely to be localized to DIG domains. Furthermore, immunoisolation of caveolin from CLMs revealed that EGF receptor activation occurs in a compartment distinct from caveolae. Similarly, using an anti-(EGF receptor) antibody, the bulk of the cellular caveolin was not co-immunoprecipitated from CLMs, thereby confirming that these two proteins reside in separate membrane domains. The deduction that caveolar signalling and EGF receptor activation occur in separable rafts argues for a multiplicity of signal transduction compartments within the plasma membrane. In addition, by demonstrating that EGF receptor activation is compartmentalized within low-density, non-caveolar regions of the plasma membrane, it is also shown that the co-localization of proteins in a CLM fraction is insufficient to prove caveolar localization.
Indexed as
Identifiers
9895306PMC1220014W2006381992What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.