ArticleMolecular pathology : MP1998
Simultaneous detection and strain differentiation of Mycobacterium tuberculosis complex in paraffin wax embedded tissues and in stained microscopic preparations.
Article in Molecular pathology : MP, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 78 citations in OpenAlex.
- Article
- Molecular typing of Mycobacterium tuberculosis: a review of current methods, databases, softwares, and analytical tools.FEMS microbiology reviews · 2025Review
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- Application of MIRU-VNTR on smear slides: a shortcut for detection of polyclonal infections in tuberculosis patients.Molecular biology reports · 2020Article
- Molecular typing methods used in studies ofIranian journal of microbiology · 2016Review
- Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.Clinical microbiology reviews · 2016Review
- SpoTyping: fast and accurate in silico Mycobacterium spoligotyping from sequence reads.Genome medicine · 2016Article
- Performance of Four Transport and Storage Systems for Molecular Detection of Multidrug-Resistant Tuberculosis.PloS one · 2015Article
- Use of conventional PCR and smear microscopy to diagnose pulmonary tuberculosis in the Amazonian rainforest area.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2014Article
- Current methods in the molecular typing of Mycobacterium tuberculosis and other mycobacteria.BioMed research international · 2014Review
- Evaluation of six different DNA extraction methods for detection of Mycobacterium tuberculosis by means of PCR-IS6110: preliminary study.BMC research notes · 2013Article
- Spoligotyping of Mycobacterium africanum, Burkina Faso.Emerging infectious diseases · 2012Article
- "Mycobacterium tilburgii" infection in two immunocompromised children: importance of molecular tools in culture-negative mycobacterial disease diagnosis.Journal of clinical microbiology · 2011Article
- DNA extracted from stained sputum smears can be used in the MTBDRplus assay.Journal of clinical microbiology · 2011Article
- Article
- African 2, a clonal complex of Mycobacterium bovis epidemiologically important in East Africa.Journal of bacteriology · 2011Article
- Changes in Mycobacterium tuberculosis genotype families over 20 years in a population-based study in Northern Malawi.PloS one · 2010Article
- Mycobacterium microti: More diverse than previously thought.Journal of clinical microbiology · 2009Article
- African 1, an epidemiologically important clonal complex of Mycobacterium bovis dominant in Mali, Nigeria, Cameroon, and Chad.Journal of bacteriology · 2009Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo detect and differentiate Mycobacterium tuberculosis simultaneously by polymerase chain reaction (PCR) in clinical samples prepared for histopathological analysis and for microscopic detection of acid fast bacteria.
methodsParaffin wax embedded tissue samples and Ziehl-Neelsen (ZN) and auramine stained microscopic preparations from culture positive tuberculosis patients were subjected to DNA extraction and amplification by PCR. PCR was performed with primers specific for direct repeats and the product was detected by hybridisation to a set of 43 different oligonucleotides, a procedure designated as "spoligotyping".
resultsMycobacterium tuberculosis complex DNA was detected in all of the 23 paraffin wax embedded tissues analysed. Strain differentiation was possible in 20 of the 23 paraffin wax embedded tissues. Mycobacterium tuberculosis complex DNA was also detected and typed in eight of 10 ZN stained microscopic preparations. The hybridisation patterns obtained from virtually all of these samples were identical to those obtained from DNA extracted from cultures.
conclusionSimultaneous detection and strain differentiation of M. tuberculosis complex bacteria is possible in clinical samples prepared by current methods for microscopic and histopathological analysis, without the need to culture. The methodology described opens the way to rapid disclosure of outbreaks in high risk settings, such as hospitals and prisons, where dissemination of tuberculosis might be very fast as a result of a high prevalence of human immunodeficiency virus infected patients.
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