ArticleProceedings of the National Academy of Sciences of the United States of America1999
Human artificial chromosomes generated by modification of a yeast artificial chromosome containing both human alpha satellite and single-copy DNA sequences.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Artificial chromosome technology and its potential application in plants.Frontiers in plant science · 2022Review
- Precise Characterization and Tracking of Stably Inherited Artificial Minichromosomes Made by Telomere-Mediated Chromosome Truncation inFrontiers in plant science · 2021Article
- Review
- Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology.Chromosoma · 2016Review
- A replicator-specific binding protein essential for site-specific initiation of DNA replication in mammalian cells.Nature communications · 2016Article
- De novo formed satellite DNA-based mammalian artificial chromosomes and their possible applications.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Article
- Strategies for designing transgenic DNA constructs.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Human artificial chromosomes for gene delivery and the development of animal models.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- De novo assembly of potential linear artificial chromosome constructs capped with expansive telomeric repeats.Plant methods · 2011Article
- Cell to cell transfer of the chromatin-packaged human beta-globin gene cluster.Nucleic acids research · 2010Article
- Artificial chromosome formation in maize (Zea mays L.).Chromosoma · 2009Article
- Manipulating transgenes using a chromosome vector.Nucleic acids research · 2009Article
- Interspecific transfer of mammalian artificial chromosomes between farm animals.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- Inactivation of a human kinetochore by specific targeting of chromatin modifiers.Developmental cell · 2008Article
- Genomic and genetic characterization of rice Cen3 reveals extensive transcription and evolutionary implications of a complex centromere.The Plant cell · 2006Article
- Human artificial chromosome vectors meet stem cells: new prospects for gene delivery.Stem cell reviews · 2006Review
- Bacterial transfer of large functional genomic DNA into human cells.Gene therapy · 2005Article
- The manipulation of chromosomes by mankind: the uses of microcell-mediated chromosome transfer.Chromosoma · 2005Review
- Artificial and engineered chromosomes: developments and prospects for gene therapy.Chromosoma · 2005Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A human artificial chromosome (HAC) vector was constructed from a 1-Mb yeast artificial chromosome (YAC) that was selected based on its size from among several YACs identified by screening a randomly chosen subset of the Centre d'Etude du Polymorphisme Humain (CEPH) (Paris) YAC library with a degenerate alpha satellite probe. This YAC, which also included non-alpha satellite DNA, was modified to contain human telomeric DNA and a putative origin of replication from the human beta-globin locus. The resultant HAC vector was introduced into human cells by lipid-mediated DNA transfection, and HACs were identified that bound the active kinetochore protein CENP-E and were mitotically stable in the absence of selection for at least 100 generations. Microdissected HACs used as fluorescence in situ hybridization probes localized to the HAC itself and not to the arms of any endogenous human chromosomes, suggesting that the HAC was not formed by telomere fragmentation. Our ability to manipulate the HAC vector by recombinant genetic methods should allow us to further define the elements necessary for mammalian chromosome function.
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