ArticleProceedings of the National Academy of Sciences of the United States of America1999
A direct role for arrestins in desensitization of the luteinizing hormone/choriogonadotropin receptor in porcine ovarian follicular membranes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 44 citations in OpenAlex.
- Noncanonical GPCR signaling arising from a PTH receptor-arrestin-Gβγ complex.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Binding between a distal C-terminus fragment of cannabinoid receptor 1 and arrestin-2.Biochemistry · 2011Article
- Interaction of a fragment of the cannabinoid CB1 receptor C-terminus with arrestin-2.FEBS letters · 2007Article
- The structural basis of arrestin-mediated regulation of G-protein-coupled receptors.Pharmacology & therapeutics · 2006Review
- Multifaceted roles of beta-arrestins in the regulation of seven-membrane-spanning receptor trafficking and signalling.The Biochemical journal · 2003Review
- Beta -Arrestin 1 down-regulation after insulin treatment is associated with supersensitization of beta 2 adrenergic receptor Galpha s signaling in 3T3-L1 adipocytes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Multiple signal transduction pathways regulate ovarian steroidogenesis.Reviews in endocrine & metabolic disorders · 2002Review
- Regulation of lutropin circulatory half-life by the mannose/N-acetylgalactosamine-4-SO4 receptor is critical for implantation in vivo.The Journal of clinical investigation · 2002Article
- The ADP ribosylation factor nucleotide exchange factor ARNO promotes beta-arrestin release necessary for luteinizing hormone/choriogonadotropin receptor desensitization.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The luteinizing hormone/choriogonadotropin (LH/CG) receptor (R) is a heptahelical R that, upon agonist binding, activates the stimulatory guanine nucleotide-binding protein (Gs) and the downstream effector adenylyl cyclase (AC). Like other G protein-coupled Rs, the LH/CG R subsequently exhibits reduced agonist-dependent effector activity, or desensitization, in response to saturating agonist. Unlike desensitization of many other G protein-coupled Rs, the in vivo desensitization response of LH/CG R-stimulated AC activity of ovarian follicles to the preovulatory surge of LH can be mimicked under cell-free conditions. Based on evidence that porcine ovarian follicular membranes unexpectedly contained beta-arrestin-1, the role of arrestins in desensitization of the LH/CG R was investigated. Results showed that neutralizing arrestin antibodies blocked the development of desensitization and that desensitization was rescued with a synthetic peptide corresponding to the antibody-binding epitope on beta-arrestin-1. These results suggest that endogenous beta-arrestin-1 participates in agonist-dependent desensitization of the LH/CG R. Addition of recombinant purified beta-arrestin-1 mimicked human chorionic gonadotrophin to promote desensitization of human chorionic gonadotrophin-stimulated AC activity, in the presence of the ATP phosphorylation antagonist adenylyl-imidodiphosphate, with an ED50 of approximately 0.1 nM. Increased levels of an 87-kDa protein reactive with glycoprotein hormone R-reactive antibody, consistent with the LH/CG R, coimmunoprecipitated with follicular membrane beta-arrestin-1 in response to LH/CG R activation compared with unactivated R. Taken together, these results show that ovarian follicles contain membrane-associated beta-arrestin-1, that beta-arrestin-1 participates in agonist-dependent desensitization of the LH/CG R, and that the trigger for beta-arrestin-1 binding to the LH/CG R appears to be R activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.