ArticleProceedings of the National Academy of Sciences of the United States of America1999
Persistence of an alternate chromatin structure at silenced loci in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Sir2 and Fun30 regulate ribosomal DNA replication timing via MCM helicase positioning and nucleosome occupancy.bioRxiv : the preprint server for biology · 2024Article
- Yeast heterochromatin regulators Sir2 and Sir3 act directly at euchromatic DNA replication origins.PLoS genetics · 2018Article
- Impact of Homologous Recombination on Silent Chromatin inGenetics · 2018Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- Nucleosomal promoter variation generates gene expression noise.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Compositional and structural analysis of selected chromosomal domains from Saccharomyces cerevisiae.Nucleic acids research · 2014Article
- In vivo role for the chromatin-remodeling enzyme SWI/SNF in the removal of promoter nucleosomes by disassembly rather than sliding.The Journal of biological chemistry · 2011Article
- Article
- The nuclear envelope in genome organization, expression and stability.Nature reviews. Molecular cell biology · 2010Review
- Long-range communication between the silencers of HMR.Molecular and cellular biology · 2008Article
- Fluorescence in situ hybridization analysis of alien genes in Agrobacterium-mediated Cry1A(b)-transformed rice.Annals of botany · 2002Article
- The Sir1 protein's association with a silenced chromosome domain.Genes & development · 2001Article
- Yeast heterochromatin is a dynamic structure that requires silencers continuously.Genes & development · 2000Article
- Telomere structure regulates the heritability of repressed subtelomeric chromatin in Saccharomyces cerevisiae.Genetics · 2000Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
In Saccharomyces cerevisiae, transcriptional repression at the HM mating-type loci and telomeres results from the formation of a heterochromatin-like structure. Silencing requires at least three Sir proteins (Sir2p-4p), which are recruited to chromatin by silencers at the HM loci and TG1-3 tracts at telomeres. Sir proteins and telomeres colocalize at the nuclear periphery, suggesting that this subnuclear position may also contribute to transcriptional repression. To evaluate the contribution of nuclear context to silencing, we developed methodology to isolate silent chromatin for analysis in vitro. Site-specific recombination was used in vivo to produce DNA rings from the silent HMR locus, and differential centrifugation was used to isolate the rings from whole-cell lysate. The partially purified rings retained many of the intracellular hallmarks of transcriptionally repressed domains. Specifically, rings from repressed strains were resistant to restriction endonuclease digestion, bore an altered DNA topology, and were associated with Sir3p. The recombination approach also was used to form rings from HMR that lacked silencers. Despite the uncoupling of these cis-acting regulatory elements, similar but nonidentical results were obtained. We conclude that an alternate chromatin structure at silent loci can persist in vitro in the absence of silencers and nuclear compartmentalization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.