ArticleMolecular and cellular biology1999
Transcription termination and 3'-End processing of the spliced leader RNA in kinetoplastids.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 69 citations in OpenAlex.
- A PCR-based method for the diagnosis ofFrontiers in microbiology · 2022Article
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- Article
- On the Possibility of an Early Evolutionary Origin for the Spliced Leader Trans-Splicing.Journal of molecular evolution · 2017Review
- Spliced leader RNA trans-splicing discovered in copepods.Scientific reports · 2015Article
- Article
- Article
- Article
- Gene expression in trypanosomatid parasites.Journal of biomedicine & biotechnology · 2010Review
- Dinoflagellate spliced leader RNA genes display a variety of sequences and genomic arrangements.Molecular biology and evolution · 2009Article
- Hypermethylated cap 4 maximizes Trypanosoma brucei translation.Molecular microbiology · 2009Article
- Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei.Molecular microbiology · 2008Article
- The promoter and transcribed regions of the Leishmania tarentolae spliced leader RNA gene array are devoid of nucleosomes.BMC microbiology · 2007Article
- Spliced leader RNA trans-splicing in dinoflagellates.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Complete cap 4 formation is not required for viability in Trypanosoma brucei.Eukaryotic cell · 2006Article
- 3'-End polishing of the kinetoplastid spliced leader RNA is performed by SNIP, a 3'-->5' exonuclease with a Motley assortment of small RNA substrates.Molecular and cellular biology · 2004Article
- Spliced-leader RNA trans splicing in a chordate, Oikopleura dioica, with a compact genome.Molecular and cellular biology · 2004Article
- Transcription initiation and termination on Leishmania major chromosome 3.Eukaryotic cell · 2004Article
- SmD1 is required for spliced leader RNA biogenesis.Eukaryotic cell · 2004Article
- trans and cis splicing in trypanosomatids: mechanism, factors, and regulation.Eukaryotic cell · 2003Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Addition of a 39-nucleotide (nt) spliced leader (SL) by trans splicing is a basic requirement for all trypanosome nuclear mRNAs. The SL RNA in Leishmania tarentolae is a 96-nt precursor transcript synthesized by a polymerase that resembles polymerase II most closely. To analyze SL RNA genesis, we mutated SL RNA intron structures and sequence elements: stem-loops II and III, the Sm-binding site, and the downstream T tract. Using an exon-tagged SL RNA gene, we examined the phenotypes produced by a second-site 10-bp linker scan mutagenic series and directed mutagenesis. Here we report that transcription is terminated by the T tract, which is common to the 3' end of all kinetoplastid SL RNA genes, and that more than six T's are required for efficient termination in vivo. We describe mutants whose SL RNAs end in the T tract or appear to lack efficient termination but can generate wild-type 3' ends. Transcriptionally active nuclear extracts show staggered products in the T tract, directed by eight or more T's. The in vivo and in vitro data suggest that SL RNA transcription termination is staggered in the T tract and is followed by nucleolytic processing to generate the mature 3' end. We show that the Sm-binding site and stem-loop III structures are necessary for correct 3'-end formation. Thus, we have defined the transcription termination element for the SL RNA gene. The termination mechanism differs from that of vertebrate small nuclear RNA genes and the SL RNA homologue in Ascaris.
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