ArticleMolecular and cellular biology1999
Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA damage checkpoint pathway.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 128 citations in OpenAlex.
- Review
- Article
- Article
- Article
- S-phase checkpoint regulations that preserve replication and chromosome integrity upon dNTP depletion.Cellular and molecular life sciences : CMLS · 2017Review
- Functional compartmentalization of Rad9 and Hus1 reveals diverse assembly of the 9-1-1 complex components during the DNA damage response in Leishmania.Molecular microbiology · 2016Article
- Genome-wide array-CGH analysis reveals YRF1 gene copy number variation that modulates genetic stability in distillery yeasts.Oncotarget · 2015Article
- Ddc2 mediates Mec1 activation through a Ddc1- or Dpb11-independent mechanism.PLoS genetics · 2014Article
- The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1.Nucleic acids research · 2014Article
- Lagging strand maturation factor Dna2 is a component of the replication checkpoint initiation machinery.Genes & development · 2013Article
- Novel checkpoint pathway organization promotes genome stability in stationary-phase yeast cells.Molecular and cellular biology · 2013Article
- Preserving Yeast Genetic Heritage through DNA Damage Checkpoint Regulation and Telomere Maintenance.Biomolecules · 2012Article
- The unstructured C-terminal tail of yeast Dpb11 (human TopBP1) protein is dispensable for DNA replication and the S phase checkpoint but required for the G2/M checkpoint.The Journal of biological chemistry · 2011Article
- Rfc5p regulates alternate RFC complex functions in sister chromatid pairing reactions in budding yeast.Cell cycle (Georgetown, Tex.) · 2010Article
- Cdc5 blocks in vivo Rad53 activity, but not in situ activity (ISA).Cell cycle (Georgetown, Tex.) · 2010Article
- A tale of two tails: activation of DNA damage checkpoint kinase Mec1/ATR by the 9-1-1 clamp and by Dpb11/TopBP1.DNA repair · 2009Review
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- Critical role of DNA checkpoints in mediating genotoxic-stress-induced filamentous growth in Candida albicans.Molecular biology of the cell · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic analysis has suggested that RAD17, RAD24, MEC3, and DDC1 play similar roles in the DNA damage checkpoint control in budding yeast. These genes are required for DNA damage-induced Rad53 phosphorylation and considered to function upstream of RAD53 in the DNA damage checkpoint pathway. Here we identify Mec3 as a protein that associates with Rad17 in a two-hybrid screen and demonstrate that Rad17 and Mec3 interact physically in vivo. The amino terminus of Rad17 is required for its interaction with Mec3, and the protein encoded by the rad17-1 allele, containing a missense mutation at the amino terminus, is defective for its interaction with Mec3 in vivo. Ddc1 interacts physically and cosediments with both Rad17 and Mec3, indicating that these three proteins form a complex. On the other hand, Rad24 is not found to associate with Rad17, Mec3, and Ddc1. DDC1 overexpression can partially suppress the phenotypes of the rad24Delta mutation: sensitivity to DNA damage, defect in the DNA damage checkpoint and decrease in DNA damage-induced phosphorylation of Rad53. Taken together, our results suggest that Rad17, Mec3, and Ddc1 form a complex which functions downstream of Rad24 in the DNA damage checkpoint pathway.
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