ArticleMolecular and cellular biology1999
Structural requirements and dynamics of mitosin-kinetochore interaction in M phase.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Centromere Protein F in Tumor Biology: Cancer's Achilles Heel.Cancer medicine · 2025Review
- CENPF (+) cancer cells promote malignant progression of early-stage TP53 mutant lung adenocarcinoma.Oncogenesis · 2025Article
- Loss of CENPF leads to developmental failure in mouse embryos.Cell cycle (Georgetown, Tex.) · 2019Article
- Miro-dependent mitochondrial pool of CENP-F and its farnesylated C-terminal domain are dispensable for normal development in mice.PLoS genetics · 2019Article
- The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases.The Journal of biological chemistry · 2018Article
- Disentangling the molecular determinants for Cenp-F localization to nuclear pores and kinetochores.EMBO reports · 2018Article
- Regulation of Cenp-F localization to nuclear pores and kinetochores.Cell cycle (Georgetown, Tex.) · 2018Article
- Dync1h1 Mutation Causes Proprioceptive Sensory Neuron Loss and Impaired Retrograde Axonal Transport of Dorsal Root Ganglion Neurons.CNS neuroscience & therapeutics · 2016Article
- A regulatory effect of INMAP on centromere proteins: antisense INMAP induces CENP-B variation and centromeric halo.PloS one · 2014Article
- Correlation between centromere protein-F autoantibodies and cancer analyzed by enzyme-linked immunosorbent assay.Molecular cancer · 2013Article
- Synthetic lethal interactions identify phenotypic "interologs" of the spindle assembly checkpoint components.Genetics · 2007Article
- Nudel modulates kinetochore association and function of cytoplasmic dynein in M phase.Molecular biology of the cell · 2007Article
- Cenp-F (mitosin) is more than a mitotic marker.Chromosoma · 2006Review
- Cytoplasmic LEK1 is a regulator of microtubule function through its interaction with the LIS1 pathway.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Silencing mitosin induces misaligned chromosomes, premature chromosome decondensation before anaphase onset, and mitotic cell death.Molecular and cellular biology · 2005Article
- Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein.The Journal of cell biology · 2004Article
- Human Nudel and NudE as regulators of cytoplasmic dynein in poleward protein transport along the mitotic spindle.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitosin is a 350-kDa human nuclear protein which transiently associates with centromeres and spindle poles in M phase. Ultrastructure studies reveal that it is located at the outer kinetochore plate. In this work, we explored the detailed structural basis and dynamics of the mitosin-kinetochore interaction. Two major regions important for targeting to centromeres were identified by analyzing different deletion mutants expressed in CHO cells: (i) the "core region" between amino acids 2792 and 2887, which was essential for the centromere localization of mitosin; and (ii) the internal repeats between residues 2094 and 2487, which cooperated with the core region to achieve strong mitosin-kinetochore interaction. The core region is characteristic of two leucine zipper motifs. Deletion of either motif abolished the centromere localization activity. In addition, Cys2864, adjacent to the second motif, was also essential for the activity of the core region. In contrast, the internal repeats alone were insufficient for centromere localization. We propose that this region may serve as a regulatory domain to facilitate interaction of the core region with the kinetochore. We showed that mitosin molecules entering nuclei after nuclear envelope breakdown (NEBD) were not assembled onto kinetochores efficiently, suggesting that the mitosin-kinetochore interaction is stabilized prior to NEBD. This result supports the idea of an ordered process for kinetochore assembly. Our data also suggest that mitosin might interact with chromatin in interphase. Evidence for coordinated regulation between the centromere-targeting and the putative chromatin-binding activities is also provided.
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