ArticleMolecular and cellular biology1999
Activator-specific requirement of yeast mediator proteins for RNA polymerase II transcriptional activation.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 87 citations in OpenAlex.
- Med9 Is a Novel eIF4E-Interacting Protein inInternational journal of molecular sciences · 2026Article
- A novel tRNA-derived fragment AS-tDR-007333 promotes the malignancy of NSCLC via the HSPB1/MED29 and ELK4/MED29 axes.Journal of hematology & oncology · 2022Article
- Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex.Biochemistry research international · 2018Article
- Article
- Toward understanding of the mechanisms of Mediator function in vivo: Focus on the preinitiation complex assembly.Transcription · 2017Review
- Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture.Genes & development · 2016Article
- Mechanisms of Mediator complex action in transcriptional activation.Cellular and molecular life sciences : CMLS · 2013Review
- High-throughput genetic and gene expression analysis of the RNAPII-CTD reveals unexpected connections to SRB10/CDK8.PLoS genetics · 2013Article
- Article
- The tail-module of yeast Mediator complex is required for telomere heterochromatin maintenance.Nucleic acids research · 2012Article
- MED29, a component of the mediator complex, possesses both oncogenic and tumor suppressive characteristics in pancreatic cancer.International journal of cancer · 2011Article
- Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization.Nucleic acids research · 2011Article
- Article
- Mechanism of Mediator recruitment by tandem Gcn4 activation domains and three Gal11 activator-binding domains.Molecular and cellular biology · 2010Article
- Mediator complex association with constitutively transcribed genes in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Functional conservation of the glutamine-rich domains of yeast Gal11 and human SRC-1 in the transactivation of glucocorticoid receptor Tau 1 in Saccharomyces cerevisiae.Molecular and cellular biology · 2008Article
- Transcriptional activation domains of the Candida albicans Gcn4p and Gal4p homologs.Eukaryotic cell · 2007Article
- Functional and physical interactions within the middle domain of the yeast mediator.Molecular genetics and genomics : MGG · 2006Article
- A functional module of yeast mediator that governs the dynamic range of heat-shock gene expression.Genetics · 2006Article
- Independent recruitment of mediator and SAGA by the activator Met4.Molecular and cellular biology · 2006Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The multisubunit Mediator complex of Saccharomyces cerevisiae is required for most RNA polymerase II (Pol II) transcription. The Mediator complex is composed of two subcomplexes, the Rgr1 and Srb4 subcomplexes, which appear to function in the reception of activator signals and the subsequent modulation of Pol II activity, respectively. In order to determine the precise composition of the Mediator complex and to explore the specific role of each Mediator protein, our goal was to identify all of the Mediator components. To this end, we cloned three previously unidentified Mediator subunits, Med9/Cse2, Med10/Nut2, and Med11, and isolated mutant forms of each of them to analyze their transcriptional defects. Differential display and Northern analyses of mRNAs from wild-type and Mediator mutant cells demonstrated an activator-specific requirement for each Mediator subunit. Med9/Cse2 and Med10/Nut2 were required, respectively, for Bas1/Bas2- and Gcn4-mediated transcription of amino acid biosynthetic genes. Gal11 was required for Gal4- and Rap1-mediated transcriptional activation. Med11 was also required specifically for MFalpha1 transcription. On the other hand, Med6 was required for all of these transcriptional activation processes. These results suggest that distinct Mediator proteins in the Rgr1 subcomplex are required for activator-specific transcriptional activation and that the activation signals mediated by these Mediator proteins converge on Med6 (or the Srb4 subcomplex) to modulate Pol II activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.