ArticleJournal of clinical microbiology1999
Quantitative analysis of mRNA as a marker for viability of Mycobacterium tuberculosis.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed, 143 citations in OpenAlex.
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- Developing biomarker assays to accelerate tuberculosis drug development: defining target product profiles.The Lancet. Microbe · 2024Review
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- The Development of a One-Step RT-qPCR for the Detection and Quantification of Viable Forms ofInternational journal of molecular sciences · 2024Article
- RNA-based amplicon sequencing is ineffective in measuring metabolic activity in environmental microbial communities.Microbiome · 2023Article
- An optimized method for purifying, detecting and quantifying Mycobacterium tuberculosis RNA from sputum for monitoring treatment response in TB patients.Scientific reports · 2022Article
- RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae).PLoS neglected tropical diseases · 2022Article
- Application of Nanopore Sequencing in the Detection of Foodborne Microorganisms.Nanomaterials (Basel, Switzerland) · 2022Review
- Feasibility of novel approaches to detect viableFrontiers in medicine · 2022Review
- Revisiting the methods for detectingAccess microbiology · 2021Review
- Direct Metatranscriptome RNA-seq and Multiplex RT-PCR Amplicon Sequencing on Nanopore MinION - Promising Strategies for Multiplex Identification of Viable Pathogens in Food.Frontiers in microbiology · 2020Article
- Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera.PloS one · 2020Article
- Heat Inactivation Renders Sputum Safe and PreservesJournal of clinical microbiology · 2019Article
- Lipoarabinomannan in sputum to detect bacterial load and treatment response in patients with pulmonary tuberculosis: Analytic validation and evaluation in two cohorts.PLoS medicine · 2019Article
- Transcriptome analysis of beta-lactamase genes in diarrheagenic Escherichia coli.Scientific reports · 2019Article
- A novel immunotherapy of Brucellosis in cows monitored non invasively through a specific biomarker.PLoS neglected tropical diseases · 2018Article
- Transcriptional Adaptation of Drug-tolerant Mycobacterium tuberculosis During Treatment of Human Tuberculosis.The Journal of infectious diseases · 2015Article
- Detection of viable plasmodium ookinetes in the midguts of anopheles coluzzi using PMA-qrtPCR.Parasites & vectors · 2015Article
- Optimization of a nucleic acid-based reporter system to detect Mycobacterium tuberculosis antibiotic sensitivity.Antimicrobial agents and chemotherapy · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Numerous assays which use conserved DNA or rRNA sequences as targets for amplification have been described for the diagnosis of tuberculosis. However, these techniques have not been applied successfully to the monitoring of therapeutic efficacy owing to the persistence of amplifiable nucleic acid beyond the point at which smears and cultures become negative. Semiquantitative analysis of rRNA has been used to reduce the time required for antimicrobial susceptibility testing of Mycobacterium tuberculosis, although growth for up to 5 days in the presence of some drugs is still required to discriminate resistant strains. The purpose of the present study was to determine whether quantitative analysis of M. tuberculosis mRNA could be used to assess bacterial viability and to illustrate the application of this technique to rapid determination of drug susceptibility. Levels of mRNA encoding the 85B protein (alpha-antigen), IS6110 DNA, and 16S rRNA were compared in parallel cultures of M. tuberculosis that were treated with either no drug, 0. 2 microg of isoniazid per ml, or 1 microg of rifampin per ml. Exposure of sensitive strains to isoniazid or rifampin for 24 h reduced the levels of 85B mRNA to <4 and <0.01%, respectively, of those present in control cultures without drug. In contrast, the levels of IS6110 DNA and 16S rRNA did not diminish over the same period. Strains which were resistant to either isoniazid or rifampin demonstrated no reduction in 85B mRNA in the presence of the drug to which they were nonresponsive. Quantitative analysis of 85B mRNA offers a potentially useful tool for the rapid determination of M. tuberculosis drug susceptibility and for the monitoring of therapeutic efficacy.
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