ArticleThe EMBO journal1999
Intranuclear targeted delivery of functional NF-kappaB by 70 kDa heat shock protein.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 67 citations in OpenAlex.
- An unprecedented alteration in mode of action of IsCT resulting its translocation into bacterial cytoplasm and inhibition of macromolecular syntheses.Scientific reports · 2015Article
- Construction of recombinant proteins for reprogramming of endangered Luxi cattle fibroblast cells.Molecular biology reports · 2012Article
- Gallus Heat shock cognate protein 70, a novel binding partner of Apoptin.Virology journal · 2011Article
- Effects of particle size on toll-like receptor 9-mediated cytokine profiles.Biomaterials · 2011Article
- Hsp70 and its molecular role in nervous system diseases.Biochemistry research international · 2011Article
- Heat shock protein 70 (Hsp70)-stimulated deoxycytidine deaminases from a human lymphoma cell but not the activation-induced cytidine deaminase (AID) from Ramos 6.4 human Burkitt's lymphoma cells.Cell stress & chaperones · 2011Article
- Activation of NK cells by extracellular heat shock protein 70 through induction of NKG2D ligands on dendritic cells.Cancer immunity · 2008Article
- Extracellular heat shock protein 70: a critical component for motoneuron survival.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Dendritic cells pulsed with hsp70-peptide complexes derived from human hepatocellular carcinoma induce specific anti-tumor immune responses.World journal of gastroenterology · 2005Article
- Clonogenicity of human leukemic cells protected from cell-lethal agents by heat shock protein 70.Cell stress & chaperones · 2005Article
- Heat shock protein 70 and the acute respiratory distress syndrome.Journal of anesthesia · 2005Review
- Administration of Hsp70 in vivo inhibits motor and sensory neuron degeneration.Cell stress & chaperones · 2004Article
- Biological applications of protein transduction technology.Trends in biotechnology · 2003Review
- Surface binding and uptake of heat shock protein 70 by antigen-presenting cells require all 3 domains of the molecule.Cell stress & chaperones · 2002Article
- Mitochondrial involvement in brain function and dysfunction: relevance to aging, neurodegenerative disorders and longevity.Neurochemical research · 2001Review
- Receptor-mediated uptake of antigen/heat shock protein complexes results in major histocompatibility complex class I antigen presentation via two distinct processing pathways.The Journal of experimental medicine · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 70 kDa heat shock protein (Hsp70) is a highly conserved, ubiquitous protein involved in chaperoning proteins to various cellular organelles. Here we show that when added exogenously to cells, Hsp70 is readily imported into both cytoplasmic and nuclear compartments in a cell-type-specific fashion. We exploited this ability of Hsp70 to deliver NF-kappaB, a key transcriptional regulator of inflammatory responses. We demonstrate that a fusion protein composed of a C-terminal Hsp70 peptide and the p50 subunit of NF-kappaB was directed into the nucleus of cells, could bind DNA specifically, and activated Igkappa expression and TNFalpha production. We therefore propose that Hsp70 can be used as a vehicle for intracytoplasmic and intranuclear delivery of proteins or DNA to modulate gene expression and thereby control immune responses.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.