ArticleJournal of the American Society for Mass Spectrometry1999
Enhanced detection of phosphopeptides in matrix-assisted laser desorption/ionization mass spectrometry using ammonium salts.
Article in Journal of the American Society for Mass Spectrometry, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Who cites it
26 citing papers in PubMed.
- Disentangle a Complex MALDI TOF Mass Spectrum of Polyethylene Glycols into Three Separate Spectra via Selective Formation of Protonated Ions and Sodium or Potassium Adducts.Journal of the American Society for Mass Spectrometry · 2022Article
- Phosphopeptide enrichment using Phos-tag technology reveals functional phosphorylation of the nucleocapsid protein of SARS-CoV-2.Journal of proteomics · 2022Article
- Glycan Imaging Mass Spectrometry: Progress in Developing Clinical Diagnostic Assays for Tissues, Biofluids, and Cells.Clinics in laboratory medicine · 2021Review
- A Rapid Array-Based Approach toJournal of proteome research · 2019Article
- Imaging Mass Spectrometry: A New Tool to Assess Molecular Underpinnings of Neurodegeneration.Metabolites · 2019Review
- Improved Detection and Fragmentation of Disulphide-Linked Peptides.Methods and protocols · 2018Article
- Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities.Methods in enzymology · 2018Article
- Improving the Sensitivity of Matrix-Assisted Ionization (MAI) Mass Spectrometry Using Ammonium Salts.Journal of the American Society for Mass Spectrometry · 2015Article
- Ammonium Ion Exchanged Zeolite for Laser Desorption/Ionization Mass Spectrometry of Phosphorylated Peptides.International journal of analytical chemistry · 2015Article
- Optimization of whole-body zebrafish sectioning methods for mass spectrometry imaging.Journal of biomolecular techniques : JBT · 2013Article
- Sperm phosphoproteomics: historical perspectives and current methodologies.Expert review of proteomics · 2012Review
- Solution additives that desalt protein ions in native mass spectrometry.Analytical chemistry · 2012Article
- Determining in vivo phosphorylation sites using mass spectrometry.Current protocols in molecular biology · 2012Article
- Regulation and identification of Na,K-ATPase alpha1 subunit phosphorylation in rat parotid acinar cells.The Journal of biological chemistry · 2010Article
- Enhanced MALDI-TOF MS analysis of phosphopeptides using an optimized DHAP/DAHC matrix.Journal of biomedicine & biotechnology · 2010Review
- Picolinamidination of phosphopeptides for MALDI-TOF-TOF mass spectrometric sequencing with enhanced sensitivity.Journal of the American Society for Mass Spectrometry · 2009Article
- Improved enrichment strategies for phosphorylated peptides on titanium dioxide using methyl esterification and pH gradient elution.Analytical biochemistry · 2008Article
- Investigating quantitation of phosphorylation using MALDI-TOF mass spectrometry.Journal of mass spectrometry : JMS · 2008Article
- Effect of the addition of diammonium citrate to alpha-cyano-4-hydroxycinnamic acid (CHCA) matrix for the detection of phosphorylated peptide in phosphorylation reactions using cell and tissue lysates.Journal of the American Society for Mass Spectrometry · 2007Article
- Development of a MALDI-TOF-MS method to identify and quantify butyrylcholinesterase inhibition resulting from exposure to organophosphate and carbamate pesticides.Journal of the American Society for Mass Spectrometry · 2007Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) has been used successfully to detect phosphorylation sites in proteins. Applications may be limited by the low response of phosphopeptides compared to nonphosphorylated peptides in MALDI MS. The addition of ammonium salts to the matrix/analyte solution substantially enhances the signal for phosphopeptides. In examples shown for equimolar mixtures, the phosphorylated peptide peaks become the largest peaks in the spectrum upon ammonium ion addition. This can allow for the identification of phosphopeptides in an unfractionated proteolytic digestion mixture. Sufficient numbers of protonated phosphopeptides can be generated such that they can be subjected to postsource decay analysis, in order to confirm the number of phosphate groups present. The approach works well with the common MALDI matrices such as alpha-cyano-4-hydroxycinnamic acid and 2,5-dihydroxybenzoic acid, and with ammonium salts such as diammonium citrate and ammonium acetate.
Indexed as
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9888183What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.