ArticleThe Journal of cell biology1999
Regulation of the actin cytoskeleton organization in yeast by a novel serine/threonine kinase Prk1p.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 97 citations in OpenAlex.
- ELM-the Eukaryotic Linear Motif resource-2024 update.Nucleic acids research · 2024Article
- Current thoughts on cellular functions of numb-associated kinases.Molecular biology reports · 2023Review
- Conserved Ark1-related kinases function in a TORC2 signaling network.Molecular biology of the cell · 2020Article
- Article
- Unbiased yeast screens identify cellular pathways affected in Niemann-Pick disease type C.Life science alliance · 2020Article
- Target of rapamycin complex 2-dependent phosphorylation of the coat protein Pan1 by Akl1 controls endocytosis dynamics inThe Journal of biological chemistry · 2018Article
- The fungal myosin I is essential for Fusarium toxisome formation.PLoS pathogens · 2018Article
- MoCAP proteins regulated by MoArk1-mediated phosphorylation coordinate endocytosis and actin dynamics to govern development and virulence of Magnaporthe oryzae.PLoS genetics · 2017Article
- TOR Complex 2-Regulated Protein Kinase Fpk1 Stimulates Endocytosis via Inhibition of Ark1/Prk1-Related Protein Kinase Akl1 in Saccharomyces cerevisiae.Molecular and cellular biology · 2017Article
- Clathrin-mediated endocytosis in budding yeast at a glance.Journal of cell science · 2016Review
- Article
- Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Casein kinase 1 promotes initiation of clathrin-mediated endocytosis.Developmental cell · 2015Article
- Targeted disruption of an EH-domain protein endocytic complex, Pan1-End3.Traffic (Copenhagen, Denmark) · 2014Article
- Article
- Article
- Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis.Journal of cell science · 2012Article
- Validation of regulated protein phosphorylation events in yeast by quantitative mass spectrometry analysis of purified proteins.Proteomics · 2012Article
- Cdc28-Cln3 phosphorylation of Sla1 regulates actin patch dynamics in different modes of fungal growth.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Normal actin cytoskeleton organization in budding yeast requires the function of the Pan1p/ End3p complex. Mutations in PAN1 and END3 cause defects in the organization of actin cytoskeleton and endocytosis. By screening for mutations that can suppress the temperature sensitivity of a pan1 mutant (pan1-4), a novel serine/threonine kinase Prk1p is now identified as a new factor regulating the actin cytoskeleton organization in yeast. The suppression of pan1-4 by prk1 requires the presence of mutant Pan1p. Although viable, the prk1 mutant is unable to maintain an asymmetric distribution of the actin cytoskeleton at 37 degreesC. Consistent with its role in the regulation of actin cytoskeleton, Prk1p localizes to the regions of cell growth and coincides with the polarized actin patches. Overexpression of the PRK1 gene in wild-type cells leads to lethality and actin cytoskeleton abnormalities similar to those exhibited by the pan1 and end3 mutants. In vitro phosphorylation assays demonstrate that Prk1p is able to phosphorylate regions of Pan1p containing the LxxQxTG repeats, including the region responsible for binding to End3p. Based on these findings, we propose that the Prk1 protein kinase regulates the actin cytoskeleton organization by modulating the activities of some actin cytoskeleton-related proteins such as Pan1p/End3p.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.