ArticleThe Journal of physiology1999
Reversible and irreversible intracellular Ca2+ spiking in single isolated human platelets.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 21 citations in OpenAlex.
- The role of phosphatidylserine on the membrane in immunity and blood coagulation.Biomarker research · 2022Review
- Targeting phosphatidylserine for Cancer therapy: prospects and challenges.Theranostics · 2020Review
- Fungal Chitin Reduces Platelet Activation Mediated via TLR8 Stimulation.Frontiers in cellular and infection microbiology · 2019Article
- Exposure of phosphatidylserine on the cell surface.Cell death and differentiation · 2016Review
- Ragged spiking of free calcium in ADP-stimulated human platelets: regulation of puff-like calcium signals in vitro and ex vivo.The Journal of physiology · 2001Article
- Reversible and irreversible intracellular Ca2+ spiking in single isolated human platelets.The Journal of physiology · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. We have developed conditions that permit long duration recordings of [Ca2+]i in single, isolated human platelets and studied the reversibility of Ca2+i spiking following activation by physiological and artificial stimuli. 2. Fura-2-loaded platelets were immobilized at the tip of a saline-filled glass pipette using gentle suction. 'Contact' activation of Ca2+i spiking was observed in a proportion (11 %) of platelets, which continued for the duration of each recording (range 8-45 min). 3. Platelets that displayed constant, resting Ca2+i levels were used to test the effects of agonists. ADP (10 microM) increased [Ca2+]i in the form of either one to two spikes followed by an elevated plateau level (60 % of cells) or multiple Ca2+ spikes of irregular amplitude (40 % of cells). ADP-induced Ca2+i mobilization was completely reversible and repeatable. 4. Thrombin (1 u ml-1) evoked Ca2+i spiking in the majority (88 %) of platelets tested, which was not inhibited by perfusion of agonist-free saline throughout the recording period (range 8-67 min). 5. The clear difference in the reversibility of activation by different stimuli may reflect the distinct roles of individual agonists in haemostasis and have important consequences in the design of treatments for thrombosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.