ArticleJournal of bacteriology1999
The mithramycin gene cluster of Streptomyces argillaceus contains a positive regulatory gene and two repeated DNA sequences that are located at both ends of the cluster.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 76 citations in OpenAlex.
- Transcriptional regulators of secondary metabolite biosynthesis in Streptomyces.World journal of microbiology & biotechnology · 2024Review
- Focus and Insights into the Synthetic Biology-Mediated Chassis of Economically Important Fungi for the Production of High-Value Metabolites.Microorganisms · 2023Review
- Metathramycin, a new bioactive aureolic acid discovered by heterologous expression of a metagenome derived biosynthetic pathway.RSC chemical biology · 2021Article
- Heterologous reconstitution of the biosynthesis pathway for 4-demethyl-premithramycinone, the aglycon of antitumor polyketide mithramycin.Microbial cell factories · 2020Article
- Disclosing the Potential of the SARP-Type Regulator PapR2 for the Activation of Antibiotic Gene Clusters in Streptomycetes.Frontiers in microbiology · 2020Article
- Comparison of Antibiotic Resistance Mechanisms in Antibiotic-Producing and Pathogenic Bacteria.Molecules (Basel, Switzerland) · 2019Review
- Manipulation of two regulatory genes for efficient production of chromomycins inJournal of biological engineering · 2018Article
- Engineered polyketides: Synergy between protein and host level engineering.Synthetic and systems biotechnology · 2017Review
- Advancement in bioprocess technology: parallels between microbial natural products and cell culture biologics.Journal of industrial microbiology & biotechnology · 2017Review
- Genetic interrelations in the actinomycin biosynthetic gene clusters ofAdvances and applications in bioinformatics and chemistry : AABC · 2017Article
- Identification by Genome Mining of a Type I Polyketide Gene Cluster fromFrontiers in microbiology · 2017Article
- Structural and functional analysis of two di-domain aromatase/cyclases from type II polyketide synthases.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Identification of a cluster-situated activator of oxytetracycline biosynthesis and manipulation of its expression for improved oxytetracycline production in Streptomyces rimosus.Microbial cell factories · 2015Article
- Complete genome sequence of producer of the glycopeptide antibiotic Aculeximycin Kutzneria albida DSM 43870T, a representative of minor genus of Pseudonocardiaceae.BMC genomics · 2014Article
- SlnM gene overexpression with different promoters on natamycin production in Streptomyces lydicus A02.Journal of industrial microbiology & biotechnology · 2014Article
- Identification and biotechnological application of novel regulatory genes involved in Streptomyces polyketide overproduction through reverse engineering strategy.BioMed research international · 2013Article
- Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Selective overproduction of the proteasome inhibitor salinosporamide A via precursor pathway regulation.Chemistry & biology · 2011Article
- Molecular insights on the biosynthesis of antitumour compounds by actinomycetes.Microbial biotechnology · 2011Review
- Characterization of the 'pristinamycin supercluster' of Streptomyces pristinaespiralis.Microbial biotechnology · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequencing of a 4.3-kb DNA region from the chromosome of Streptomyces argillaceus, a mithramycin producer, revealed the presence of two open reading frames (ORFs). The first one (orfA) codes for a protein that resembles several transport proteins. The second one (mtmR) codes for a protein similar to positive regulators involved in antibiotic biosynthesis (DnrI, SnoA, ActII-orf4, CcaR, and RedD) belonging to the Streptomyces antibiotic regulatory protein (SARP) family. Both ORFs are separated by a 1.9-kb, apparently noncoding region. Replacement of the mtmR region by an antibiotic resistance cassette completely abolished mithramycin biosynthesis. Expression of mtmR in a high-copy-number vector in S. argillaceus caused a 16-fold increase in mithramycin production. The mtmR gene restored actinorhodin production in Streptomyces coelicolor JF1 mutant, in which the actinorhodin-specific activator ActII-orf4 is inactive, and also stimulated actinorhodin production by Streptomyces lividans TK21. A 241-bp region located 1.9 kb upstream of mtmR was found to be repeated approximately 50 kb downstream of mtmR at the other end of the mithramycin gene cluster. A model to explain a possible route for the acquisition of the mithramycin gene cluster by S. argillaceus is proposed.
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