ArticleJournal of bacteriology1999
Discontinuous occurrence of the hsp70 (dnaK) gene among Archaea and sequence features of HSP70 suggest a novel outlook on phylogenies inferred from this protein.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 107 citations in OpenAlex.
- Nature's economy as blueprint: regional overlap & block-based algorithm for protein design.Frontiers in bioinformatics · 2026Article
- Protein folding in vitro and in the cell: From a solitary journey to a team effort.Biophysical chemistry · 2022Review
- Allosteric Inter-Domain Contacts in Bacterial Hsp70 Are Located in Regions That Avoid Insertion and Deletion Events.International journal of molecular sciences · 2022Article
- Protein Family Content Uncovers Lineage Relationships and Bacterial Pathway Maintenance Mechanisms in DPANN Archaea.Frontiers in microbiology · 2021Article
- New insights into marine group III Euryarchaeota, from dark to light.The ISME journal · 2017Article
- Neuroprotective strategies for traumatic brain injury: improving clinical translation.International journal of molecular sciences · 2014Review
- ATP-driven molecular chaperone machines.Biopolymers · 2013Review
- Identification and characterization of a novel human methyltransferase modulating Hsp70 protein function through lysine methylation.The Journal of biological chemistry · 2013Article
- Horizontal gene transfer of a chloroplast DnaJ-Fer protein to Thaumarchaeota and the evolutionary history of the DnaK chaperone system in Archaea.BMC evolutionary biology · 2012Article
- Over-expression of HSP70 attenuates caspase-dependent and caspase-independent pathways and inhibits neuronal apoptosis.Journal of neurochemistry · 2012Article
- Reassessment of the lineage fusion hypothesis for the origin of double membrane bacteria.PloS one · 2011Article
- The origins of phagocytosis and eukaryogenesis.Biology direct · 2009Article
- Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library.Cell stress & chaperones · 2009Article
- Evolution of a protein-folding machine: genomic and evolutionary analyses reveal three lineages of the archaeal hsp70(dnaK) gene.Journal of molecular evolution · 2006Article
- Predicted highly expressed genes in archaeal genomes.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Molecular characterization of the group II chaperonin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3.Extremophiles : life under extreme conditions · 2005Article
- Novel chaperonins in a prokaryote.Journal of molecular evolution · 2005Article
- The archaeal Sec-dependent protein translocation pathway.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2004Review
- The consistent phylogenetic signal in genome trees revealed by reducing the impact of noise.Journal of molecular evolution · 2004Article
- Cloning, expression, and biochemical characterization of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthase from the hyperthermophilic bacterium Aquifex pyrophilus.Extremophiles : life under extreme conditions · 2003Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Occurrence of the hsp70 (dnaK) gene was investigated in various members of the domain Archaea comprising both euryarchaeotes and crenarchaeotes and in the hyperthermophilic bacteria Aquifex pyrophilus and Thermotoga maritima representing the deepest offshoots in phylogenetic trees of bacterial 16S rRNA sequences. The gene was not detected in 8 of 10 archaea examined but was found in A. pyrophilus and T. maritima, from which it was cloned and sequenced. Comparative analyses of the HSP70 amino acid sequences encoded in these genes, and others in the databases, showed that (i) in accordance with the vicinities seen in rRNA-based trees, the proteins from A. pyrophilus and T. maritima form a thermophilic cluster with that from the green nonsulfur bacterium Thermomicrobium roseum and are unrelated to their counterparts from gram-positive bacteria, proteobacteria/mitochondria, chlamydiae/spirochetes, deinococci, and cyanobacteria/chloroplasts; (ii) the T. maritima HSP70 clusters with the homologues from the archaea Methanobacterium thermoautotrophicum and Thermoplasma acidophilum, in contrast to the postulated unique kinship between archaea and gram-positive bacteria; and (iii) there are exceptions to the reported association between an insert in HSP70 and gram negativity, or vice versa, absence of insert and gram positivity. Notably, the HSP70 from T. maritima lacks the insert, although T. maritima is phylogenetically unrelated to the gram-positive bacteria. These results, along with the absence of hsp70 (dnaK) in various archaea and its presence in others, suggest that (i) different taxa retained either one or the other of two hsp70 (dnaK) versions (with or without insert), regardless of phylogenetic position; and (ii) archaea are aboriginally devoid of hsp70 (dnaK), and those that have it must have received it from phylogenetically diverse bacteria via lateral gene transfer events that did not involve replacement of an endogenous hsp70 (dnaK) gene.
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