ArticleJournal of virology1999
Double-stranded linear duck hepatitis B virus (DHBV) stably integrates at a higher frequency than wild-type DHBV in LMH chicken hepatoma cells.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Multiplex gene editing suppresses random integration of hepatitis B virus DNA in chronically infected liver.Molecular therapy. Nucleic acids · 2026Article
- Detection of HBV DNA integration in plasma cell-free DNA of different HBV diseases utilizing DNA capture strategy.Virologica Sinica · 2024Article
- Hepatitis B Virus DNA Integration and Clonal Expansion of Hepatocytes in the Chronically Infected Liver.Viruses · 2021Review
- Hepatitis B Virus DNA Integration Occurs Early in the Viral Life Cycle in anJournal of virology · 2018Article
- Review
- Review
- HBV DNA Integration and Clonal Hepatocyte Expansion in Chronic Hepatitis B Patients Considered Immune Tolerant.Gastroenterology · 2016Article
- Molecular biology of hepatitis B virus infection.Virology · 2015Review
- Animal models and the molecular biology of hepadnavirus infection.Cold Spring Harbor perspectives in medicine · 2015Review
- Human plasmacytoid dendritic cells sense lymphocytic choriomeningitis virus-infected cells in vitro.Journal of virology · 2014Article
- Clonal expansion of normal-appearing human hepatocytes during chronic hepatitis B virus infection.Journal of virology · 2010Article
- Article
- Detection of clonally expanded hepatocytes in chimpanzees with chronic hepatitis B virus infection.Journal of virology · 2009Article
- The amount of hepatocyte turnover that occurred during resolution of transient hepadnavirus infections was lower when virus replication was inhibited with entecavir.Journal of virology · 2009Article
- Immune selection during chronic hepadnavirus infection.Hepatology international · 2008Article
- Integration of hepatitis B virus DNA into chromosomal DNA during acute hepatitis B.World journal of gastroenterology · 2005Article
- Clonal expansion of hepatocytes during chronic woodchuck hepatitis virus infection.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Genomic DNA double-strand breaks are targets for hepadnaviral DNA integration.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Residual integrated viral DNA after hepadnavirus clearance by nucleoside analog therapy.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Presence of replicating virus in recombinant hepadnavirus stocks results from recombination and can be eliminated by the use of a packaging cell line.Journal of virology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Integration of hepadnavirus DNAs into host chromosomes can have oncogenic consequences. Analysis of host-viral DNA junctions of DHBV identified the terminally duplicated r region of the viral genome as a hotspot for integration. Since the r region is present on the 5' and 3' ends of double-stranded linear (DSL) hepadnavirus DNAs, these molecules have been implicated as integration precursors. We have produced a LMH chicken hepatoma cell line (LMH 66-1 DSL) which replicates exclusively DSL duck hepatitis B virus (DHBV) DNA. To test whether linear DHBV DNAs integrate more frequently than the wild type open circular DHBV DNAs, we have characterized the integration frequency in LMH 66-1 DSL cells by using a subcloning approach. This approach revealed that 83% of the LMH 66-1 DSL subclones contained new integrations, compared to only 16% of subclones from LMH-D2 cells replicating wild-type open circular DHBV DNA. Also, a higher percentage of the LMH 66-1 DSL subclones contained two or more new integrations. Mathematical analysis suggests that the DSL DHBV DNAs integrated stably once every three generations during subcloning whereas wild-type DHBV integrated only once every four to five generations. Cloning and sequencing of new integrations confirmed the r region as a preferred integration site for linear DHBV DNA molecules. One DHBV integrant was associated with a small deletion of chromosomal DNA, and another DHBV integrant occurred in a telomeric repeat sequence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.