ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Capsaicin binds to the intracellular domain of the capsaicin-activated ion channel.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
82 citing papers in PubMed, 278 citations in OpenAlex.
- Bibliometric analysis of recent research on the association between TRPV1 and inflammation.Channels (Austin, Tex.) · 2023Article
- Resiniferatoxin: Nature's Precision Medicine to Silence TRPV1-Positive Afferents.International journal of molecular sciences · 2023Review
- A journey from molecule to physiology andFrontiers in pharmacology · 2023Review
- Role and Modulation of TRPV1 in Mammalian Spermatozoa: An Updated Review.International journal of molecular sciences · 2021Review
- Injectable Capsaicin for the Management of Pain Due to Osteoarthritis.Molecules (Basel, Switzerland) · 2021Review
- Capsaicin-Sensitive Sensory Nerves and the TRPV1 Ion Channel in Cardiac Physiology and Pathologies.International journal of molecular sciences · 2020Review
- Combination of a Rapidly Penetrating Agonist and a Slowly Penetrating Antagonist Affords Agonist Action of Limited Duration at the Cellular Level.Biomolecules & therapeutics · 2019Article
- Characterization of Membrane Patch-Ion Channel Probes for Scanning Ion Conductance Microscopy.Small (Weinheim an der Bergstrasse, Germany) · 2018Article
- Novel Radiolabeled Vanilloid with Enhanced Specificity for Human Transient Receptor Potential Vanilloid 1 (TRPV1).Journal of medicinal chemistry · 2017Article
- Transient Receptor Potential Vanilloid 1 Regulates Mitochondrial Membrane Potential and Myocardial Reperfusion Injury.Journal of the American Heart Association · 2016Article
- A pro-nociceptive phenotype unmasked in mice lacking fatty-acid amide hydrolase.Molecular pain · 2016Article
- Metabolomics meets functional assays: coupling LC-MS and microfluidic cell-based receptor-ligand analyses.Metabolomics : Official journal of the Metabolomic Society · 2016Article
- Photoswitchable fatty acids enable optical control of TRPV1.Nature communications · 2015Article
- Capsaicin interaction with TRPV1 channels in a lipid bilayer: molecular dynamics simulation.Biophysical journal · 2015Article
- Targeting the transient receptor potential vanilloid type 1 (TRPV1) assembly domain attenuates inflammation-induced hypersensitivity.The Journal of biological chemistry · 2014Article
- Effect of chirality and lipophilicity in the functional activity of evodiamine and its analogues at TRPV1 channels.British journal of pharmacology · 2014Article
- Cannabidiol enhances microglial phagocytosis via transient receptor potential (TRP) channel activation.British journal of pharmacology · 2014Article
- Regulation of TRPV1 ion channel by phosphoinositide (4,5)-bisphosphate: the role of membrane asymmetry.The Journal of biological chemistry · 2014Article
- Intracellular long-chain acyl CoAs activate TRPV1 channels.PloS one · 2014Article
- High-resolution views of TRPV1 and their implications for the TRP channel superfamily.Handbook of experimental pharmacology · 2014Review
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Capsaicin (CAP) excites small sensory neurons, causing pain, neurogenic inflammation, and other visceral reflexes. These effects have been proposed to be the result of CAP activation of a nonselective cation current. It is generally assumed that CAP binds to an extracellular domain of the membrane receptor. However, the exact binding site is not known because of the lipophilic nature of CAP. To determine whether the binding domain is extracellular or intracellular, we tested the effect of a synthetic water-soluble CAP analog, DA-5018.HCl, on current activation. CAP activated the 45 pS (at -60 mV) nonselective cation channel from either side of the membrane. However, DA-5018.HCl, which had a greater potency and efficacy than CAP, activated the channels only from the cytosolic side of the patch membrane in a capsazepine, a CAP receptor antagonist, reversible manner. When applied extracellularly, DA-5018. HCl did not, but CAP did, activate whole-cell currents in sensory neurons, as well as in oocytes expressing vanilloid receptor 1, a recently cloned CAP receptor. Hydrogen ions, reported as a possible endogenous activator of cation current, failed to elicit any current when acidic medium (pH 5.0-6.0) was applied intracellularly, indicating that H+ does not mediate the CAP effect. These results indicate that CAP and its analog bind to the cytosolic domain of the CAP receptor and suggest that an endogenous CAP-like substance other than H+ may be present in the cell.
Indexed as
Identifiers
9880573PMC6782213W2128604157What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.