ArticlePlant physiology1999
Age-induced protein modifications and increased proteolysis in potato seed-tubers
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 67 citations in OpenAlex.
- Functional divergence of annotated l-isoaspartateThe Journal of biological chemistry · 2019Article
- A high-throughput peptidomic strategy to decipher the molecular diversity of cyclic cysteine-rich peptides.Scientific reports · 2016Article
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- QTLs for seed vigor-related traits identified in maize seeds germinated under artificial aging conditions.PloS one · 2014Article
- Characterization of Solanum tuberosum multicystatin and the significance of core domains.The Plant cell · 2013Article
- Article
- Molecular analysis of maize cystatin expression as fusion product in Escherichia coli.Physiology and molecular biology of plants : an international journal of functional plant biology · 2012Article
- Enhanced seed viability and lipid compositional changes during natural ageing by suppressing phospholipase Dα in soybean seed.Plant biotechnology journal · 2012Article
- Crystal structure of tarocystatin-papain complex: implications for the inhibition property of group-2 phytocystatins.Planta · 2011Article
- Article
- Drought tolerance of juvenile and mature leaves of a deciduous dwarf shrub Vaccinium myrtillus L. in a boreal environment.Protoplasma · 2010Article
- Article
- Characterization of Solanum tuberosum multicystatin and its structural comparison with other cystatins.The Plant cell · 2009Article
- Potato (Solanum tuberosum L.) tuber ageing induces changes in the proteome and antioxidants associated with the sprouting pattern.Journal of experimental botany · 2009Article
- Article
- Arabidopsis Protein Repair L-Isoaspartyl Methyltransferases: Predominant Activities at Lethal Temperatures.Physiologia plantarum · 2006Article
- Article
- Distinct patterns of expression but similar biochemical properties of protein L-isoaspartyl methyltransferase in higher plants.Plant physiology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Long-term aging of potato (Solanum tuberosum) seed-tubers resulted in a loss of patatin (40 kD) and a cysteine-proteinase inhibitor, potato multicystatin (PMC), as well as an increase in the activities of 84-, 95-, and 125-kD proteinases. Highly active, additional proteinases (75, 90, and 100 kD) appeared in the oldest tubers. Over 90% of the total proteolytic activity in aged tubers was sensitive to trans-epoxysuccinyl-L-leucylamido (4-guanidino) butane or leupeptin, whereas pepstatin was the most effective inhibitor of proteinases in young tubers. Proteinases in aged tubers were also inhibited by crude extracts or purified PMC from young tubers, suggesting that the loss of PMC was responsible for the age-induced increase in proteinase activity. Nonenzymatic oxidation, glycation, and deamidation of proteins were enhanced by aging. Aged tubers developed "daughter" tubers that contained 3-fold more protein than "mother" tubers, with a polypeptide profile consistent with that of young tubers. Although PMC and patatin were absent from the older mother tubers, both proteins were expressed in the daughter tubers, indicating that aging did not compromise the efficacy of genes encoding PMC and patatin. Unlike the mother tubers, proteinase activity in daughter tubers was undetectable. Our results indicate that tuber aging nonenzymatically modifies proteins, which enhances their susceptibility to breakdown; we also identify a role for PMC in regulating protein turnover in potato tubers.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.