ArticleMolecular biology of the cell1999
Disruption of a dynamin homologue affects endocytosis, organelle morphology, and cytokinesis in Dictyostelium discoideum.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 109 citations in OpenAlex.
- The dynamin-related proteinFrontiers in plant science · 2026Article
- Expanding ring-shaped cleavage furrows in multinucleate cells.Molecular biology of the cell · 2023Article
- Review
- The Amoebal Model for Macropinocytosis.Sub-cellular biochemistry · 2022Article
- Identification of GOLPH3 Partners inCells · 2021Article
- Review
- Article
- Dynamin-Like Protein B ofCells · 2019Article
- Review
- Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis.Molecular biology of the cell · 2018Article
- Evolution of cytokinesis-related protein localization during the emergence of multicellularity in volvocine green algae.BMC evolutionary biology · 2017Article
- WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- An ancestral bacterial division system is widespread in eukaryotic mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Ancient dynamin segments capture early stages of host-mitochondrial integration.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cytokinesis in animal cells.Cold Spring Harbor perspectives in biology · 2015Review
- Reversible membrane pearling in live cells upon destruction of the actin cortex.Biophysical journal · 2014Article
- Mechanisms of mitochondrial fission and fusion.Cold Spring Harbor perspectives in biology · 2013Review
- Anterior PAR proteins function during cytokinesis and maintain DYN-1 at the cleavage furrow in Caenorhabditis elegans.Cytoskeleton (Hoboken, N.J.) · 2012Article
- The balance in the delivery of ER components and the vacuolar proton pump to the phagosome depends on myosin IK in Dictyostelium.Molecular & cellular proteomics : MCP · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The identification and functional characterization of Dictyostelium discoideum dynamin A, a protein composed of 853 amino acids that shares up to 44% sequence identity with other dynamin-related proteins, is described. Dynamin A is present during all stages of D. discoideum development and is found predominantly in the cytosolic fraction and in association with endosomal and postlysosomal vacuoles. Overexpression of the protein has no adverse effect on the cells, whereas depletion of dynamin A by gene-targeting techniques leads to multiple and complex phenotypic changes. Cells lacking a functional copy of dymA show alterations of mitochondrial, nuclear, and endosomal morphology and a defect in fluid-phase uptake. They also become multinucleated due to a failure to complete normal cytokinesis. These pleiotropic effects of dynamin A depletion can be rescued by complementation with the cloned gene. Morphological studies using cells producing green fluorescent protein-dynamin A revealed that dynamin A associates with punctate cytoplasmic vesicles. Double labeling with vacuolin, a marker of a postlysosomal compartment in D. discoideum, showed an almost complete colocalization of vacuolin and dynamin A. Our results suggest that that dynamin A is likely to function in membrane trafficking processes along the endo-lysosomal pathway of D. discoideum but not at the plasma membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.